Toro L, Zazzarelli A
Can J Biochem. 1978 Dec;56(12):1120-3. doi: 10.1139/o78-176.
A new recirculation reactor for kinetic studies of immobilized enzyme is proposed. The reactor consists of two tubes in the same thermostatting jacket. One of the tubes is filled with the immobilized enzyme, the other is filled only with the support. During the approach to steady-state temperature, the circulation is carried out only in the inactive tube. Subsequently, when temperature is stable, the active reactor is used and the solution is not recirculated.
