Diffusional limitations in liquid-phase reactions catalyzed by enzymes immobilized on porous supports: sucrose inversion by beta-fructooxidase immobilized on IRA-93 resin.

An experimental procedure has been proposed to study liquid-phase reactions catalyzed by enzymes immobilized on porous supports. The kinetic behavior can be assessed by experimental runs which allow a separate evaluation of the internal diffusion, the surface kinetics, and the fluid-particle mass transfer. The method has been applied in the study of sucrose inversion by beta-fructooxidase immobilized on IRA-93 resin.