骨髓纤维化综合征患者脾脏中细胞脱氧核糖核酸聚合酶及一种逆转录酶的纯化、生化特性鉴定和血清学分析
Purification, biochemical characterization and serological analysis of cellular deoxyribonucleic acid polymerases and a reverse transcriptase from spleen of a patient with myelofibrotic syndrome.
作者信息
Chandra P, Steel L K
出版信息
Biochem J. 1977 Dec 1;167(3):513-24. doi: 10.1042/bj1670513f.
Abstract
The present study describes the separation and purification of a reverse transcriptase and cellular DNA polymerases from the human spleen of a patient with myelofibrotic syndrome. The specific requirements with respect to bivalent cations and template-primers for DNA polymerase-alpha, DNA polymerase-beta and DNA polymerase-gamma, as well as for the reverse transcriptase, are reported. Sedimentation-velocity measurements of the purified enzymes gave values of 150000, 40000, 100000 and 70000 daltons for DNA polymerase-alpha DNA polymerase-beta, DNA polymerase-gamma and the reverse transcriptase respectively. Serological studies have shown that the reverse transcriptase from human spleen is not antigenically related to cellular DNA polymerase-alpha, -beta or -gamma, but is antigenically related to reverse transcriptase from simian sarcoma virus and gibbon-ape leukaemia virus.
摘要
本研究描述了从一名骨髓纤维化综合征患者的人脾脏中分离和纯化逆转录酶及细胞DNA聚合酶的过程。报告了DNA聚合酶α、DNA聚合酶β、DNA聚合酶γ以及逆转录酶对二价阳离子和模板引物的具体要求。对纯化酶的沉降速度测量结果显示，DNA聚合酶α、DNA聚合酶β、DNA聚合酶γ和逆转录酶的分子量分别为150000、40000、100000和70000道尔顿。血清学研究表明，人脾脏中的逆转录酶与细胞DNA聚合酶α、β或γ无抗原相关性，但与猿猴肉瘤病毒和长臂猿白血病病毒的逆转录酶有抗原相关性。
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