Culić M, Saponjić J, Janković B, Udović S, Popović S, Rakić L
Institute for Biological Research, University of Belgrade, Yugoslavia.
Neurosci Lett. 1995 Aug 18;196(1-2):49-52. doi: 10.1016/0304-3940(95)11835-k.
The effect of the adrenergic neurotoxin N-(chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) on electroencephalographic (EEG) activity was studied in the model of epilepsy induced by systemic application of penicillin (1,000,000 IU/kg, i.p). DSP4 (50 mg/kg, i.p.) was administrated to male Wistar rats, while the control animals were rats from the same litters. EEG activity was recorded in acute and chronic experiments 3 or 4 weeks after DSP4 treatment, before and after penicillin administration. Occasional locus coeruleus (LC) stimulation served as an electrophysiological test of DSP4 toxic effect. EEG power spectra in DSP4 treated animals showed a tendency to be greater in lower frequency bands than in controls before penicillin administration; there was almost no effect of electrical LC stimulation, regardless on penicillin treatment. In the model of epilepsy, the mean total EEG power spectra were greater in the period of 135-330 min after penicillin administration, as well as during 345-540 min, in DSP4 treated animals as compared to the controls. It seems that neurotoxin DSP4 is an optimal tool for studying the removal of LC influence in the acute model of epilepsy. It is also suggested that norepinephrine (NE) may have a modulatory role in the systemic penicillin epilepsy.
在全身注射青霉素（1000000 IU/kg，腹腔注射）诱导的癫痫模型中，研究了肾上腺素能神经毒素N-（氯乙基）-N-乙基-2-溴苄胺（DSP4）对脑电图（EEG）活动的影响。将DSP4（50 mg/kg，腹腔注射）给予雄性Wistar大鼠，而对照动物为来自同一窝的大鼠。在DSP4治疗后3或4周的急性和慢性实验中，在青霉素给药前后记录EEG活动。偶尔刺激蓝斑（LC）作为DSP4毒性作用的电生理测试。在青霉素给药前，DSP4处理的动物的EEG功率谱在低频带中显示出比对照更大的趋势；无论青霉素治疗如何，电刺激LC几乎没有影响。在癫痫模型中，与对照相比，DSP4处理的动物在青霉素给药后135 - 330分钟期间以及345 - 540分钟期间的平均总EEG功率谱更大。神经毒素DSP4似乎是研究在急性癫痫模型中消除LC影响的最佳工具。还表明去甲肾上腺素（NE）可能在全身青霉素诱导癫痫中具有调节作用。
