Kaur C, Ling E A
Department of Anatomy, Faculty of Medicine, National University of Singapore, Kent Ridge, Singapore.
Neurosci Lett. 1995 Aug 25;196(3):165-8. doi: 10.1016/0304-3940(95)11866-u.
The epiplexus cells in postnatal rats were markedly reduced in number and immunoreactivity for OX-42, OX-18 and ED1 following subcutaneous injections of dexamethasone. This was especially evident in rats receiving two or three successive injections of dexamethasone and killed at the age of 4 or 7 days. At 14 and 21 days, the cells did not exhibit any striking difference from their corresponding controls in terms of cell number and immunoreactivity for the above antibodies. Occasional epiplexus cells were labelled with the antibody OX-6 in both groups of rats sacrificed at different time points. In rats receiving dexamethasone coupled with rhodamine isothiocyanate (RhIc), the epiplexus cells, though fewer in number than the corresponding controls, emitted bright fluorescence. It is concluded that the reduction of epiplexus cells following dexamethasone injections is due to the suppression of their precursor cells, i.e. monocytes. The phagocytic activity of the persisting epiplexus cells did not appear to be abolished by dexamethasone as evidenced by their uptake of RhIc. Our results suggest that the effects of dexamethasone are reversible.
皮下注射地塞米松后，新生大鼠脉络丛细胞数量显著减少，且OX - 42、OX - 18和ED1的免疫反应性降低。这在接受两次或三次连续地塞米松注射并在4或7日龄处死的大鼠中尤为明显。在14日龄和21日龄时，这些细胞在数量以及上述抗体的免疫反应性方面与相应对照组相比无显著差异。在不同时间点处死的两组大鼠中，偶尔有脉络丛细胞被OX - 6抗体标记。在接受地塞米松与异硫氰酸罗丹明（RhIc）结合物注射的大鼠中，脉络丛细胞数量虽比相应对照组少，但发出明亮荧光。得出的结论是，注射地塞米松后脉络丛细胞数量减少是由于其前体细胞即单核细胞受到抑制。持续存在的脉络丛细胞对RhIc的摄取表明，地塞米松似乎并未消除其吞噬活性。我们的结果表明，地塞米松的作用是可逆的。
