一种用cDNA筛选产生的探针分离oligo(dT)引发的cDNA克隆的简化方法。 - Suppr | 超能文献

A simplified approach for isolating oligo(dT) primed cDNA clones with probes generated by cDNA selection.

作者信息

Xu H, Wei H, Kolluri R, Weissman S M

机构信息

Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06536-0812, USA.

出版信息

Nucleic Acids Res. 1995 Nov 11;23(21):4528-9. doi: 10.1093/nar/23.21.4528.

DOI:10.1093/nar/23.21.4528

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307416/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c05/307416/48c9da0b8150/nar00021-0310-a.jpg

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本文引用的文献

1

cDNA selection from total yeast DNA containing YACs.从含有酵母人工染色体（YACs）的酵母总DNA中进行互补DNA（cDNA）筛选。

Nucleic Acids Res. 1993 Sep 11;21(18):4422-3. doi: 10.1093/nar/21.18.4422.

2

Genes in one megabase of the HLA class I region.HLA I类区域中一个百万碱基范围内的基因。

Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11870-4. doi: 10.1073/pnas.90.24.11870.

3

Application of cDNA selection techniques to regions of the human MHC.cDNA筛选技术在人类主要组织相容性复合体区域的应用。

Genomics. 1993 Sep;17(3):575-81. doi: 10.1006/geno.1993.1374.

4

Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids.Charon BS（阳性）和（阴性），用于构建定向cDNA文库并高效转移至质粒的通用λ噬菌体载体。

Nucleic Acids Res. 1988 Sep 12;16(17):8739. doi: 10.1093/nar/16.17.8739.

5

pSH4: a mammalian cDNA expression vector.pSH4：一种哺乳动物cDNA表达载体。

Nucleic Acids Res. 1990 Jun 25;18(12):3668. doi: 10.1093/nar/18.12.3668.

6

Direct selection: a method for the isolation of cDNAs encoded by large genomic regions.直接筛选：一种分离由大基因组区域编码的cDNA的方法。

Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9628-32. doi: 10.1073/pnas.88.21.9628.

7

cDNA selection: efficient PCR approach for the selection of cDNAs encoded in large chromosomal DNA fragments.cDNA筛选：用于筛选大染色体DNA片段中编码的cDNA的高效PCR方法。

Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9623-7. doi: 10.1073/pnas.88.21.9623.