Ersfeld K, Craig P S
Department of Biological Sciences, University of Salford, UK.
Parasitol Res. 1995;81(5):382-7. doi: 10.1007/BF00931498.
A cDNA clone of Echinococcus granulosus has been isolated from an expression library screened with sera from cystic echinococcosis patients. The deduced amino acid sequence shows 56% homology to the heavy chain of human ferritin. E. granulosus ferritin contains 173 amino acid residues and has a calculated molecular weight of 19830 Da and a statistical isoelectric point of 7.6. Functionally important amino acid residues of the ferroxidase centre are conserved in comparison with other ferritins. In vitro-translated E. granulosus ferritin was tested for its diagnostic potential by immunoprecipitation. The antigenic reactivity exhibited a good potential for the further development of E. granulosus ferritin as an immunodiagnostic tool for human hydatidosis.
从用囊性棘球蚴病患者血清筛选的表达文库中分离出了细粒棘球绦虫的一个互补DNA（cDNA）克隆。推导的氨基酸序列与人铁蛋白重链显示出56%的同源性。细粒棘球绦虫铁蛋白含有173个氨基酸残基，计算分子量为19830道尔顿，统计等电点为7.6。与其他铁蛋白相比，铁氧化酶中心功能上重要的氨基酸残基是保守的。通过免疫沉淀测试了体外翻译的细粒棘球绦虫铁蛋白的诊断潜力。该抗原反应性显示出细粒棘球绦虫铁蛋白作为人类包虫病免疫诊断工具进一步开发的良好潜力。
