Lyubchenko Y L, Jacobs B L, Lindsay S M, Stasiak A
Department of Microbiology, Arizona State University, Tempe 85287-2701, USA.
Scanning Microsc. 1995 Sep;9(3):705-24; discussion 724-7.
Recent data on the AFM studies of nucleoprotein complexes of different types are reviewed in this paper. The first section describes the progress in the sample preparation methods for AFM studies of nucleic acids and nucleoprotein complexes. The second part of this paper reviews AFM data on studies of complexes of DNA with regulatory proteins. These studies include two different types of DNA distortion induced by proteins binding: local bending of DNA at sites of protein binding and formation of large loops due to protein-protein interactions between molecules bound to distant sites along the DNA molecules (DNA looping). The prospects for use of AFM for physical mapping of genomes are discussed in this section as well. The third part of the paper reviews data on studies of complexes of DNA with non-sequence specific binding proteins. Special emphasis is given to studies of chromatin which have resulted in progress in the understanding of structure of native chromatin fiber. In this section, novel data on AFM studies of RecA-DNA filaments and complexes of dsRNA with the dsRNA-specific protein p25 are also presented. Discussion of the substrate preparation procedures in relation to the AFM studies of nucleoprotein complexes is given in the final section.
本文综述了近期不同类型核蛋白复合物的原子力显微镜（AFM）研究数据。第一部分描述了用于核酸和核蛋白复合物AFM研究的样品制备方法的进展。本文的第二部分综述了关于DNA与调节蛋白复合物研究的AFM数据。这些研究包括蛋白质结合诱导的两种不同类型的DNA畸变：蛋白质结合位点处DNA的局部弯曲以及由于沿DNA分子与远处位点结合的分子之间的蛋白质-蛋白质相互作用而形成的大环（DNA环化）。本节还讨论了使用AFM进行基因组物理图谱绘制的前景。本文的第三部分综述了关于DNA与非序列特异性结合蛋白复合物研究的数据。特别强调了染色质研究，这些研究在理解天然染色质纤维结构方面取得了进展。在本节中，还展示了关于RecA-DNA细丝和双链RNA与双链RNA特异性蛋白p25复合物的AFM研究的新数据。最后一部分讨论了与核蛋白复合物AFM研究相关的底物制备程序。
