Gallery E D, Rowe J, Campbell S, Hawkins T
Department of Renal Medicine, Royal North Shore Hospital, St. Leonard's, New South Wales, Australia.
Am J Obstet Gynecol. 1995 Nov;173(5):1557-62. doi: 10.1016/0002-9378(95)90649-5.
An increasing amount of circumstantial evidence points to the maternal endothelial cell as centrally involved in the syndrome of preeclampsia. The purposes of this study were (1) to compare the secretion of vasoactive substances by maternal decidual endothelial cells with that of umbilical vein endothelial cells, widely used as a surrogate for endothelial cells in general, and (2) to compare secretion of the same vasoactive substances by decidual endothelial cells from normal and preeclamptic pregnancies.
Endothelial cells were isolated from umbilical veins and from decidual biopsy specimens collected at cesarean section delivery from both normal and preeclamptic women. Cells were maintained in culture until passage 2, when secretion by the three endothelial cell populations of the vasodilators prostaglandin E2 and prostacyclin and the vasoconstrictor endothelin-1 was examined. In addition to control incubations, their responses to stimulation and suppression of secretion were compared.
In control incubations normal decidual endothelial cells secreted lower amounts of prostacyclin, prostaglandin E2, and endothelin than did human umbilical vein endothelial cells. All cell types had qualitatively similar responses to the stimuli used, but quantitatively different responses were noted between human umbilical vein endothelial cells and normal decidual endothelial cells for all metabolites examined. Preeclamptic decidual endothelial cells secreted significantly more prostaglandin E2 than did normal decidual endothelial cells in response to stimulation.
We have delineated levels of secretion of vasoactive substances by human late pregnancy decidual endothelial cells and their responses to manipulation of secretory pathways. There are differences between this endothelial cell population and human umbilical vein endothelial cells (which are widely used as a surrogate for maternal endothelial cells). The differences between normal and preeclamptic decidual endothelial cells in prostaglandin E2 secretion may point to altered regulation of arachidonic acid metabolic pathways in preeclampsia.
越来越多的间接证据表明，母体内皮细胞在子痫前期综合征中起核心作用。本研究的目的是：（1）比较母体蜕膜内皮细胞与脐静脉内皮细胞（广泛用作一般内皮细胞的替代物）血管活性物质的分泌情况；（2）比较正常妊娠和子痫前期妊娠的蜕膜内皮细胞中相同血管活性物质的分泌情况。
从脐静脉以及正常和子痫前期妇女剖宫产分娩时采集的蜕膜活检标本中分离内皮细胞。细胞培养至第2代，检测三种内皮细胞群体中血管舒张剂前列腺素E2和前列环素以及血管收缩剂内皮素-1的分泌情况。除了对照孵育外，还比较了它们对分泌刺激和抑制的反应。
在对照孵育中，正常蜕膜内皮细胞分泌的前列环素、前列腺素E2和内皮素比人脐静脉内皮细胞少。所有细胞类型对所用刺激的反应在性质上相似，但在所有检测的代谢产物中，人脐静脉内皮细胞和正常蜕膜内皮细胞之间的反应在数量上有所不同。子痫前期蜕膜内皮细胞在受到刺激时分泌的前列腺素E2明显多于正常蜕膜内皮细胞。
我们已经描述了人类妊娠晚期蜕膜内皮细胞血管活性物质的分泌水平及其对分泌途径调控的反应。该内皮细胞群体与人类脐静脉内皮细胞（广泛用作母体内皮细胞的替代物）之间存在差异。正常和子痫前期蜕膜内皮细胞在前列腺素E2分泌方面的差异可能表明子痫前期花生四烯酸代谢途径的调节发生了改变。
