Rowe J, Campbell S, Gallery E D
Department of Renal Medicine, Sydney University at Royal North Shore Hospital, St Leonards, 2065, New South Wales, Australia.
J Soc Gynecol Investig. 2001 Jan-Feb;8(1):32-8.
To examine the effect of plasma from preeclamptic women on production of the vasoactive substances prostacyclin, thromboxane, nitric oxide, and cyclic guanosine monophosphate (cGMP) by decidual endothelial cells; to determine any effects on cell growth and health; and to examine whether cells from preeclamptic women are activated compared with cells from normal women.
Decidual endothelial cells from normal and preeclamptic women were incubated for 24 hours in media containing 10% plasma from preeclamptic women or matched normal women. Prostacyclin and thromboxane production was measured, as was nitric oxide and cGMP production after a further 45-minute generation period in 2% test plasma. Cell numbers and lactate dehydrogenase release were also determined.
In plasma from preeclamptic women, cells grew significantly faster (P <.05), prostacyclin production was increased (P <.05), and lactate dehydrogenase release was reduced (P <.01). Production of thromboxane, nitric oxide, and cGMP was not significantly affected. Decidual endothelial cells from preeclamptic women had increased growth (P <.0001) and produced more prostacyclin (P <.05) and nitric oxide (P <.001) than normal decidual endothelial cells. There were highly significant correlations between prostacyclin and thromboxane production for incubations in plasmas from preeclamptic women and between background levels of prostacyclin in each plasma from preeclamptic women and the prostacyclin produced in incubations containing that plasma (P <.0001).
We found that plasma from preeclamptic women contained a factor that stimulated endothelial cell growth and regulated production of related amounts of prostacyclin and thromboxane. The plasma level of this factor appeared to be related to background levels of prostacyclin. The results also indicated that decidual endothelial cells from preeclamptic women were in a relatively activated state.
研究子痫前期患者血浆对蜕膜内皮细胞生成血管活性物质前列环素、血栓素、一氧化氮和环磷酸鸟苷（cGMP）的影响；确定其对细胞生长和健康的任何作用；并研究与正常女性细胞相比，子痫前期患者的细胞是否被激活。
将正常和子痫前期患者的蜕膜内皮细胞在含有10%子痫前期患者血浆或匹配的正常女性血浆的培养基中孵育24小时。测量前列环素和血栓素的生成，以及在2%测试血浆中再孵育45分钟后的一氧化氮和cGMP生成。还测定了细胞数量和乳酸脱氢酶释放量。
在子痫前期患者血浆中，细胞生长明显加快（P<.05），前列环素生成增加（P<.05），乳酸脱氢酶释放减少（P<.01）。血栓素、一氧化氮和cGMP的生成未受明显影响。子痫前期患者的蜕膜内皮细胞比正常蜕膜内皮细胞生长加快（P<.0001），生成更多的前列环素（P<.05）和一氧化氮（P<.001）。子痫前期患者血浆孵育时前列环素和血栓素生成之间，以及子痫前期患者每种血浆中前列环素的背景水平与含有该血浆的孵育中生成的前列环素之间存在高度显著的相关性（P<.0001）。
我们发现子痫前期患者血浆中含有一种因子，可刺激内皮细胞生长并调节相关量的前列环素和血栓素的生成。该因子的血浆水平似乎与前列环素的背景水平有关。结果还表明，子痫前期患者的蜕膜内皮细胞处于相对激活状态。
