Gallery E D, Rowe J, Campbell S, Hawkins T
Department of Renal Medicine, Royal North Shore Hospital, St. Leonard's, New South Wales, Australia.
Am J Obstet Gynecol. 1995 Sep;173(3 Pt 1):918-23. doi: 10.1016/0002-9378(95)90366-6.
Increasing circumstantial evidence suggests that the maternal endothelial cell is centrally involved in the syndrome of preeclampsia, and a number of reports have described the presence of a factor(s) that alters endothelial cell function in serum from women with preeclampsia. We have previously described differences between endothelial cells from the decidual vascular bed and those from the umbilical vein. The purposes of this study were (1) to examine the effect of serum from normal and preeclamptic women on secretion of vasoactive substances by maternal decidual endothelial cells, (2) to compare these results with those from umbilical vein endothelial cells, widely used as a surrogate for endothelial cells in general, (3) to compare responses to these sera by decidual endothelial cells from normal and preeclamptic pregnancies, and (4) to determine whether these responses are amplified by preincubation in test sera.
Endothelial cells were isolated from umbilical veins and from decidual biopsy specimens collected at caesarean section delivery, from both normal and preeclamptic women. Cells were maintained in culture until passage 2, when secretion by the three endothelial cell populations of the vasodilator prostacyclin (measured as its stable metabolite, 6-keto-prostaglandin F1 alpha) and the vasoconstrictor endothelin-1 was compared in the presence of serum from preeclamptic or gestational age-matched normal pregnant women.
Prostacyclin secretion by all endothelial cell populations was higher in the presence of serum from preeclamptic women than in medium containing serum from gestational age-matched normal pregnant women. Values for endothelin were not significantly different in cells incubated in serum from normal or preeclamptic women. Preincubation of decidual cells from preeclamptic women in test serum, particularly in preeclamptic serum, resulted in more marked stimulation of prostacyclin secretion.
Preeclamptic serum contains a factor(s) that stimulates prostanoid secretion from endothelial cells. This effect was observed in both umbilical vein and decidual cells. Cells from preeclamptic women were more susceptible to perturbation of their secretion by this factor. Serum from preeclamptic women did not specifically affect endothelin-1 secretion.
越来越多的间接证据表明，母体内皮细胞在子痫前期综合征中起核心作用，并且有许多报告描述了子痫前期女性血清中存在改变内皮细胞功能的一种或多种因子。我们之前描述了蜕膜血管床内皮细胞与脐静脉内皮细胞之间的差异。本研究的目的是：（1）研究正常和子痫前期女性血清对母体蜕膜内皮细胞血管活性物质分泌的影响；（2）将这些结果与广泛用作一般内皮细胞替代物的脐静脉内皮细胞的结果进行比较；（3）比较正常和子痫前期妊娠的蜕膜内皮细胞对这些血清的反应；（4）确定在测试血清中预孵育是否会放大这些反应。
从正常和子痫前期女性剖宫产分娩时采集的脐静脉和蜕膜活检标本中分离内皮细胞。细胞培养至第2代，比较在子痫前期或孕周匹配的正常孕妇血清存在下，三种内皮细胞群体中血管舒张剂前列环素（以其稳定代谢物6-酮-前列腺素F1α测量）和血管收缩剂内皮素-1的分泌情况。
在子痫前期女性血清存在下，所有内皮细胞群体的前列环素分泌均高于含有孕周匹配的正常孕妇血清的培养基中的分泌。在正常或子痫前期女性血清中孵育的细胞中，内皮素值无显著差异。子痫前期女性的蜕膜细胞在测试血清中预孵育，尤其是子痫前期血清中预孵育，导致前列环素分泌受到更明显的刺激。
子痫前期血清中含有一种刺激内皮细胞前列腺素分泌的因子。在脐静脉和蜕膜细胞中均观察到这种效应。子痫前期女性的细胞对该因子引起的分泌扰动更敏感。子痫前期女性血清未特异性影响内皮素-1的分泌。
