Chen F T, Evangelista R A
Advanced Technology Center, Beckman Instruments Inc., Fullerton, California 92634, USA.
Anal Biochem. 1995 Sep 20;230(2):273-80. doi: 10.1006/abio.1995.1474.
A method of analysis of isomers of mono- and oligosaccharides derivatized with 9-aminopyrene-1,4,6-trisulfonate (APTS) by capillary electrophoresis (CE) in simple buffer systems is presented. The derivatization chemistry is performed at 75 degrees C for 1 h for most mono- and oligosaccharides. Sialyllactose was derivatized at 37 degrees C for 15 h without significant desialylation. Most of the APTS derivatized isomers of mono- and oligosaccharides are resolved in buffers such as acetate (pH 5.0), 3-(N-morpholino)-propanesulfonic acid (pH 7.0), and phosphate (pH 7.4). The mechanism of CE separation using the above buffers is based on the differences in hydrodynamic volume of the derivatized species and is different from that in borate or high pH buffer.
本文介绍了一种在简单缓冲体系中，通过毛细管电泳（CE）分析用9-氨基芘-1,4,6-三磺酸盐（APTS）衍生化的单糖和寡糖异构体的方法。对于大多数单糖和寡糖，衍生化反应在75℃下进行1小时。唾液乳糖在37℃下衍生化15小时，且无明显的去唾液酸化现象。大多数经APTS衍生化的单糖和寡糖异构体在醋酸盐（pH 5.0）、3-（N-吗啉代）丙磺酸（pH 7.0）和磷酸盐（pH 7.4）缓冲液中得以分离。使用上述缓冲液进行CE分离的机制基于衍生化物种流体动力学体积的差异，与在硼酸盐或高pH缓冲液中的机制不同。
