基于聚合酶链式反应（PCR）利用磁珠构建消减cDNA文库 - Suppr

PCR-based construction of subtractive cDNA library using magnetic beads.

作者信息

Sharma P, Lönneborg A, Stougaard P

机构信息

Norwegian Forest Research Institute, Plant Molecular Biology Laboratory, As.

出版信息

Biotechniques. 1993 Oct;15(4):610, 612.

PMID:7504496

Abstract

摘要

相似文献

PCR-based construction of subtractive cDNA library using magnetic beads.

Biotechniques. 1993 Oct;15(4):610, 612.

Construction of subtractive cDNA libraries from limited amounts of mRNA and multiple cycles of subtraction.

Biotechniques. 1993 Oct;15(4):654-6, 658-9.

Superparamagnetic beads: applications of solid-phase RT-PCR.

Am Biotechnol Lab. 1994 May;12(6):12-4.

cDNA generation on paramagnetic beads.

Methods Mol Biol. 2003;221:25-31. doi: 10.1385/1-59259-359-3:25.

Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance PCR and magnetic beads.

Methods Mol Biol. 2002;192:99-108. doi: 10.1385/1-59259-177-9:099.

Subtractive cloning of differential genes using RNA-PCR.

Methods Mol Biol. 2003;221:253-9. doi: 10.1385/1-59259-359-3:253.

Subtractive hybridization strategy using paramagnetic oligo(dT) beads and PCR.

Biotechniques. 1996 Mar;20(3):413-9. doi: 10.2144/19962003413.

RT-PCR in cDNA library construction.

Methods Mol Biol. 2002;193:349-61. doi: 10.1385/1-59259-283-X:349.

[Construction and preliminary identification of subtracted cDNA library of leukemia cell line K562].

Ai Zheng. 2005 May;24(5):631-3.

Subtractive cDNA cloning using magnetic beads and PCR.

Methods Mol Biol. 1997;67:371-87. doi: 10.1385/0-89603-483-6:371.

引用本文的文献

The plant virus Tomato Spotted Wilt Tospovirus activates the immune system of its main insect vector, Frankliniella occidentalis.

J Virol. 2004 May;78(10):4976-82. doi: 10.1128/jvi.78.10.4976-4982.2004.

Molecular interactions between the specialist herbivore Manduca sexta (lepidoptera, sphingidae) and its natural host Nicotiana attenuata: V. microarray analysis and further characterization of large-scale changes in herbivore-induced mRNAs.

Plant Physiol. 2003 Apr;131(4):1877-93. doi: 10.1104/pp.102.018176.

Isolation of the first putative peroxidase cDNA from a conifer and the local and systemic accumulation of related proteins upon pathogen infection.

Plant Mol Biol. 2001 Oct;47(3):423-35. doi: 10.1023/a:1011615328684.

Measurement of bacterial gene expression in vivo.

Philos Trans R Soc Lond B Biol Sci. 2000 May 29;355(1397):601-11. doi: 10.1098/rstb.2000.0601.

Subtractive hybridization of cDNA from small amounts of plant tissue.

Mol Biotechnol. 1997 Aug;8(1):7-12. doi: 10.1007/BF02762335.

Isolation and characterization of a cDNA encoding a plant defensin-like protein from roots of Norway spruce.

Plant Mol Biol. 1996 Jun;31(3):707-12. doi: 10.1007/BF00042244.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

PCR-based construction of subtractive cDNA library using magnetic beads.

作者信息

机构信息

出版信息

相似文献

引用本文的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

相似文献

引用本文的文献