从有限量的mRNA构建消减cDNA文库及多轮消减
Construction of subtractive cDNA libraries from limited amounts of mRNA and multiple cycles of subtraction.
作者信息
López-Fernández L A, del Mazo J
机构信息
Centro de Investigaciones Biológicas, C.S.I.C., Madrid, Spain.
出版信息
Biotechniques. 1993 Oct;15(4):654-6, 658-9.
PMID:7504497
Abstract
A new strategy to construct subtractive cDNA libraries was developed. The method was based on multiple subtraction cycles from small amounts of RNA using paramagnetic technology. An additional application of this technique is the potential to recover (and reuse) the same mRNAs or the single-stranded cDNA from the solid paramagnetic support. The result was assessed in the generation of a specific prepuberal mouse testis library.
摘要
一种构建消减cDNA文库的新策略被开发出来。该方法基于使用顺磁技术从少量RNA进行多次消减循环。这项技术的另一个应用潜力是能够从固体顺磁支持物上回收（并重复使用）相同的mRNA或单链cDNA。在构建一个特定的青春期前小鼠睾丸文库中对结果进行了评估。
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