蓝舌病毒：用于血清学诊断的病毒抗原的生产与研究

Bluetongue virus: production and study of viral antigen for serological diagnosis.

作者信息

Arita G M, Gatti M S, Deak J G, de Castro A F

机构信息

Laboratorio de Referência Animal, LARA/Campinas, MARA, São Paulo, Brazil.

出版信息

J Virol Methods. 1993 Oct;44(2-3):281-6. doi: 10.1016/0166-0934(93)90063-w.

DOI:10.1016/0166-0934(93)90063-w

PMID:7505285

Abstract

A soluble antigen, produced from the culture supernatant of VERO cells infected with bluetongue virus serotype 4 (BTV-S4) and concentrated by sequential ultrafiltration with membranes with cut-off values 10(3) and 25 x 10(3) NMWP, showed complete identity to standard antigens when compared by agar gel immunodiffusion (AGID) and SDS-PAGE profiles, revealing that the main protein component responsible for the AGID reaction has a molecular weight of about 60 kDa corresponding probably to the NS1 protein.

摘要

一种可溶性抗原，由感染蓝舌病病毒血清型4（BTV-S4）的VERO细胞培养上清液产生，并通过使用截留分子量为10³和25×10³ NMWP的膜进行连续超滤浓缩，通过琼脂凝胶免疫扩散（AGID）和SDS-PAGE图谱比较时，与标准抗原显示出完全相同，表明负责AGID反应的主要蛋白质成分的分子量约为60 kDa，可能对应于NS1蛋白。