Bajorath J, Stenkamp R, Aruffo A
Bristol-Myers Squibb, Pharmaceutical Research Institute, Seattle, Washington 98121.
Protein Sci. 1993 Nov;2(11):1798-810. doi: 10.1002/pro.5560021103.
We describe how to build protein models from structural templates. Methods to identify structural similarities between proteins in cases of significant, moderate to low, or virtually absent sequence similarity are discussed. The detection and evaluation of structural relationships is emphasized as a central aspect of protein modeling, distinct from the more technical aspects of model building. Computational techniques to generate and complement comparative protein models are also reviewed. Two examples, P-selectin and gp39, are presented to illustrate the derivation of protein model structures and their use in experimental studies.
我们描述了如何从结构模板构建蛋白质模型。讨论了在序列相似性显著、中等至低或几乎不存在的情况下识别蛋白质之间结构相似性的方法。强调了结构关系的检测和评估是蛋白质建模的核心方面,这与模型构建的更多技术方面不同。还综述了生成和完善比较蛋白质模型的计算技术。给出了两个例子,P-选择素和gp39,以说明蛋白质模型结构的推导及其在实验研究中的应用。