Mamta B, Rajam M V
Department of Genetics, University of Delhi South Campus, Benito Juarez Marg, New Delhi, 110021 India.
Physiol Mol Biol Plants. 2017 Jul;23(3):487-501. doi: 10.1007/s12298-017-0443-x. Epub 2017 Apr 29.
The insect pests are big threat in meeting the food demands for future generation. The present pest control strategies, including the existing transgenic approaches show certain limitations and are not completely successful in limiting the insect pests. However, the sequence-specific gene silencing via RNA interference (RNAi) holds a great promise for effective management of agricultural pests. RNAi is naturally occurring conserved process responsible for gene regulation and defense against pathogens. The efficacy of RNAi varies among different insect orders and also depends upon various factors, including the target gene selection, method of dsRNAs delivery, expression of dsRNAs and presence of off-target effects. RNAi-mediated silencing of different insect genes involved in various physiological processes was found to be detrimental to insects growth, development and survival. In this article, we have reviewed the potential of RNAi-based strategies for effective management of insect pests. We have also discussed the various parameters, which are to be considered for host-induced RNAi-mediated control of insect pests without producing any effect on non-target organisms and environment.
害虫对满足后代的粮食需求构成巨大威胁。目前的害虫防治策略，包括现有的转基因方法，都存在一定的局限性，在限制害虫方面并不完全成功。然而，通过RNA干扰（RNAi）进行的序列特异性基因沉默在有效管理农业害虫方面具有巨大潜力。RNAi是一种自然发生的保守过程，负责基因调控和抵御病原体。RNAi的效果在不同昆虫目之间有所不同，并且还取决于各种因素，包括靶基因的选择、双链RNA（dsRNAs）的递送方法、dsRNAs的表达以及脱靶效应的存在。人们发现，RNAi介导的参与各种生理过程的不同昆虫基因的沉默对昆虫的生长、发育和生存有害。在本文中，我们综述了基于RNAi的策略在有效管理害虫方面的潜力。我们还讨论了在宿主诱导的RNAi介导的害虫防治中，在不影响非靶标生物和环境的情况下需要考虑的各种参数。
