三碘甲状腺原氨酸对小鼠胎肝细胞甲胎蛋白和白蛋白产生的调节作用。

The regulatory role of tri-iodothyronine on the production of alpha-fetoprotein and albumin by mouse fetal liver cells.

作者信息

Anteby E, Shpan P, Dushnik M, Zvang A, Zer T, Ben-Neriah Z, Yagel S

机构信息

Department of Obstetrics and Gynaecology, Hadassah Mt. Scopus, Jerusalem, Israel.

出版信息

Hum Reprod. 1993 Oct;8(10):1576-8. doi: 10.1093/oxfordjournals.humrep.a137894.

DOI:10.1093/oxfordjournals.humrep.a137894

PMID:7507935

Abstract

The purpose of this study was to assess possible effects of tri-iodothyronine (T3) on the production of alpha fetoprotein (AFP) and albumin by mouse fetal liver cells. AFP from serum-free conditioned media of TIB73 mouse fetal liver cells was measured by immunoradiometric assay and albumin was measured by chromogenic assay. The expression of mRNAs was quantified by Northern blotting analysis. A marked inhibition of AFP secretion was found as well as an increase in albumin produced by T3 in a dose-dependent manner. The effects of T3 AFP and albumin secretion paralleled the effects of T3 on the steady-state expression of mRNAs encoding albumin and AFP. These data may point to a possible role of T3 in the transcriptional switch from AFP to albumin during fetal development and may explain the observation of high levels of AFP in cases of congenital hypothyroidism.

摘要

本研究的目的是评估三碘甲状腺原氨酸（T3）对小鼠胎儿肝细胞产生甲胎蛋白（AFP）和白蛋白的可能影响。通过免疫放射分析测定来自TIB73小鼠胎儿肝细胞无血清条件培养基中的AFP，并用显色法测定白蛋白。通过Northern印迹分析对mRNA的表达进行定量。发现T3以剂量依赖的方式显著抑制AFP分泌并增加白蛋白的产生。T3对AFP和白蛋白分泌的影响与T3对编码白蛋白和AFP的mRNA稳态表达的影响平行。这些数据可能表明T3在胎儿发育过程中从AFP到白蛋白的转录转换中可能发挥作用，并可能解释先天性甲状腺功能减退症病例中AFP水平升高的现象。