Phillips P, Helm K F
Department of Pathology, Pennsylvania State University College of Medicine, Hershey.
J Cutan Pathol. 1993 Oct;20(5):424-8. doi: 10.1111/j.1600-0560.1993.tb00665.x.
Histologic differentiation of keratoacanthoma (KA) and squamous cell carcinoma (SCC) is often difficult despite well-delineated histopathologic criteria. This has prompted a search for more objective methods to differentiate these two lesions. In the present study, we immunohistochemically examined the distribution of proliferating cell nuclear antigen (PCNA)-positive cells in 11 cases of KA, 7 cutaneous SCC, and 2 atypical squamous proliferations (for which a definitive diagnosis could not be made on routine histology) using a commercially prepared anti-PCNA monoclonal antibody. We found PCNA-positive cells predominantly in the periphery of squamous nests in KA. In contrast, SCC showed a diffuse staining pattern with PCNA-positive cells seen throughout squamous nests. Determining the pattern of PCNA-positive cells is easy, does not require cell counting, and may provide additional histochemical data facilitating the distinction between KA and SCC.
尽管有明确的组织病理学标准，但角化棘皮瘤（KA）和鳞状细胞癌（SCC）的组织学鉴别往往很困难。这促使人们寻找更客观的方法来区分这两种病变。在本研究中，我们使用市售的抗增殖细胞核抗原（PCNA）单克隆抗体，对11例KA、7例皮肤SCC和2例非典型鳞状增生（常规组织学无法做出明确诊断）中PCNA阳性细胞的分布进行了免疫组织化学检查。我们发现KA中PCNA阳性细胞主要位于鳞状巢的周边。相比之下，SCC呈现弥漫性染色模式，在整个鳞状巢中均可见PCNA阳性细胞。确定PCNA阳性细胞的模式很容易，不需要细胞计数，并且可能提供有助于区分KA和SCC的额外组织化学数据。
