短杆菌肽与脂质相互作用的溶剂历史依赖性：拉曼光谱和红外光谱研究
Solvent history dependence of gramicidin-lipid interactions: a Raman and infrared spectroscopic study.
作者信息
Bouchard M, Auger M
机构信息
Département de chimie, Université Laval, Québec, Canada.
出版信息
Biophys J. 1993 Dec;65(6):2484-92. doi: 10.1016/S0006-3495(93)81300-6.
Abstract
We have investigated the interactions between gramicidin and a model membrane composed of one phospholipid, dimyristoylphosphatidylcholine, as a function of the cosolubilization solvent and incubation time used in the sample preparation. Three organic solvents have been used; trifluoroethanol, a mixture of methanol/chloroform (1:1 v/v), and ethanol. Using Fourier transform infrared spectroscopy, we have demonstrated that the conformation adopted by gramicidin in the membrane is dependent upon the cosolubilization solvent used, and, only with trifluoroethanol, it is possible to incorporate gramicidin entirely as a beta 6.3-helix. Moreover, Raman spectroscopy results indicate that the orientation of the tryptophan side chains in gramicidin and their interaction with the hydrocarbon chains and the carbonyl groups of the lipids are also dependent on the cosolubilization solvent. On the other hand, the effect of the incorporation of gramicidin on the thermotropism of the lipid bilayer was found to be dependent upon the conformation of gramicidin in the lipid bilayers.
摘要
我们研究了短杆菌肽与由一种磷脂——二肉豆蔻酰磷脂酰胆碱构成的模型膜之间的相互作用，该相互作用是样品制备中使用的共溶解溶剂和孵育时间的函数。使用了三种有机溶剂：三氟乙醇、甲醇/氯仿（1:1 v/v）混合物和乙醇。通过傅里叶变换红外光谱，我们证明了短杆菌肽在膜中所采用的构象取决于所使用的共溶解溶剂，并且只有使用三氟乙醇时，才有可能将短杆菌肽完全以β 6.3-螺旋形式掺入。此外，拉曼光谱结果表明，短杆菌肽中色氨酸侧链的取向及其与脂质的烃链和羰基的相互作用也取决于共溶解溶剂。另一方面，发现掺入短杆菌肽对脂质双层热致性的影响取决于短杆菌肽在脂质双层中的构象。
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