用溴甲酚紫进行荧光染色：一种作为杀伤毒素活性测定方法而开发的快速测定酵母细胞死亡数的方法。
Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.
作者信息
Kurzweilová H, Sigler K
机构信息
Institute of Microbiology, Czech Academy of Sciences, Prague.
出版信息
Yeast. 1993 Nov;9(11):1207-11. doi: 10.1002/yea.320091107.
PMID:7509098
Abstract
A method is described for detecting yeast cells with plasma membrane damage, based on cell staining with bromocresol purple (BCP) which has a convenient fluorescence after entering the cells at pH below 5.2. The method was used to determine the activity of Saccharomyces cerevisiae pore-forming killer toxin K1 in commonly used lethal units. The BCP test is rapid and as precise as the plating test.
摘要
本文描述了一种检测具有质膜损伤的酵母细胞的方法，该方法基于用溴甲酚紫（BCP）对细胞进行染色，在pH低于5.2时进入细胞后BCP会产生便利的荧光。该方法用于测定酿酒酵母成孔杀伤毒素K1在常用致死单位中的活性。BCP测试快速且与平板测试一样精确。
Zotero 插件