Kurzweilová H, Sigler K
Institute of Microbiology, Czech Academy of Sciences, Prague.
Experientia. 1995 Jan 15;51(1):26-8.
A new rapid assay of the yeast killer toxin K1 activity in Saccharomyces cerevisiae, the BCP (bromocresol purple) fluorescence test, was compared with two standard methods, the plating test and the well test. The BCP test is as precise as the plating test and more sensitive than the well test. Under suitable conditions, the test is universally applicable for determining the proportion of cells with a damaged plasma membrane in a population. A procedure was developed for calculating the results of all three tests in terms of a single well-defined unit (lethal or killer unit, LU).
一种用于快速检测酿酒酵母中杀伤毒素K1活性的新方法——BCP（溴甲酚紫）荧光试验，与两种标准方法即平板试验和杯碟试验进行了比较。BCP试验与平板试验一样精确，且比杯碟试验更灵敏。在合适的条件下，该试验普遍适用于测定群体中质膜受损细胞的比例。已制定出一种程序，可根据单一明确的单位（致死或杀伤单位，LU）来计算这三种试验的结果。
