Bowrin V, Brissette R, Tsung K, Inouye M
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey at Rutgers, Piscataway 08854.
FEMS Microbiol Lett. 1994 Jan 1;115(1):1-6. doi: 10.1111/j.1574-6968.1994.tb06605.x.
Overproduction of the alpha subunit of RNA polymerase in Escherichia coli resulted in inhibition of transcription of two osmoregulated porin genes, ompF and ompC, but not of constitutively expressed housekeeping genes. Overproduction of the sigma subunit did not have any inhibitory effects. The specific inhibitory effect of the alpha subunit was also found to depend upon the OmpR protein, the transcriptional activator for ompF and ompC. These results are in general agreement with other biochemical and genetic evidence suggesting that the alpha subunit is the subunit of RNA polymerase that directly interacts with certain transcriptional activators to initiate transcription.
大肠杆菌中RNA聚合酶α亚基的过量表达导致两个渗透压调节孔蛋白基因ompF和ompC的转录受到抑制，但组成型表达的管家基因不受影响。σ亚基的过量表达没有任何抑制作用。还发现α亚基的特异性抑制作用依赖于OmpR蛋白，即ompF和ompC的转录激活因子。这些结果与其他生化和遗传学证据总体一致，表明α亚基是RNA聚合酶中直接与某些转录激活因子相互作用以启动转录的亚基。
