Matsuyama S, Mizushima S
Institute of Applied Microbiology, University of Tokyo, Japan.
J Mol Biol. 1987 Jun 20;195(4):847-53. doi: 10.1016/0022-2836(87)90489-x.
The expression of the ompF and ompC genes that code for major outer-membrane proteins of Escherichia coli is positively regulated by the products of the ompR and envZ genes. Recently, we isolated the ompR77 mutation, which suppresses the envZ11 mutation. In this work, a novel mutation that interferes with the suppression of the envZ11 mutation by ompR77 was isolated. The mutation was located in the rpoA gene that codes for the alpha subunit of DNA-dependent RNA polymerase. These results suggest that an interaction between the positive regulators and RNA polymerase is involved in the initiation of transcription of the ompF and ompC genes. In addition, the results suggest that during transcription the RNA polymerase migrates along DNA strands with the alpha subunit facing backward and the beta beta' subunits facing forward.
编码大肠杆菌主要外膜蛋白的ompF和ompC基因的表达受ompR和envZ基因产物的正向调控。最近，我们分离出了ompR77突变，它能抑制envZ11突变。在这项工作中，我们分离出了一种新的突变，该突变干扰了ompR77对envZ11突变的抑制作用。该突变位于编码依赖DNA的RNA聚合酶α亚基的rpoA基因中。这些结果表明，正向调节因子与RNA聚合酶之间的相互作用参与了ompF和ompC基因转录的起始。此外，结果表明在转录过程中，RNA聚合酶沿着DNA链移动，α亚基向后，ββ'亚基向前。
