Renaud F, Oliver L, Desset S, Tassin J, Romquin N, Courtois Y, Laurent M
Unité de Recherches Gérontologiques, INSERM U.118, affiliée CNRS, Association Claude-Bernard, Paris, France.
J Cell Physiol. 1994 Mar;158(3):435-43. doi: 10.1002/jcp.1041580307.
Exogenously administrated acidic FGF modulates the proliferation of several cell types, controls cell differentiation, and promotes cell survival. Most cells that are sensitive to exogenous aFGF are also capable of expressing it at very low levels. Thus in order to establish the role of endogenous aFGF as a mitogenic, differentiation, or survival factor, we studied the regulation of aFGF expression by evaluating the level of mRNA by PCR amplification and the concentration of protein by Enzyme Immuno Assay (EIA). In the lens, the amount of aFGF transcripts in nondividing cells of the central epithelium and in the differentiated fiber cells located at the periphery of the lens is similar, suggesting that endogenous aFGF is not involved with lens differentiation. In cultures, depending on the growth conditions, the endogenous aFGF expressed by Bovine Epithelial Lens (BEL) cells is subject to modulation. Cells arrested either by contact inhibition or by serum deprivation express more aFGF transcripts and protein than in exponentially growing cells, implying that endogenous aFGF has no mitogenic role under these conditions. In serum-deprived cells, the addition of specific aFGF antisense primers inhibits endogenous aFGF expression and leads to the death of these cells. These results associated with the higher expression of aFGF in nondividing BEL cells, suggesting that, contrary to exogenous aFGF, endogenous aFGF is not a mitogenic factor but a survival factor.
外源性给予的酸性成纤维细胞生长因子（FGF）可调节多种细胞类型的增殖，控制细胞分化，并促进细胞存活。大多数对外源性酸性FGF敏感的细胞也能够以极低水平表达它。因此，为了确定内源性酸性FGF作为促有丝分裂、分化或存活因子的作用，我们通过聚合酶链反应（PCR）扩增评估mRNA水平，并通过酶免疫测定（EIA）检测蛋白质浓度，来研究酸性FGF表达的调控。在晶状体中，中央上皮的非分裂细胞和位于晶状体周边的分化纤维细胞中酸性FGF转录本的量相似，这表明内源性酸性FGF与晶状体分化无关。在培养物中，根据生长条件的不同，牛晶状体上皮（BEL）细胞表达的内源性酸性FGF会受到调控。通过接触抑制或血清剥夺而停滞生长的细胞，比指数生长的细胞表达更多的酸性FGF转录本和蛋白质，这意味着在这些条件下内源性酸性FGF没有促有丝分裂作用。在血清剥夺的细胞中，添加特异性酸性FGF反义引物会抑制内源性酸性FGF的表达，并导致这些细胞死亡。这些结果与非分裂BEL细胞中酸性FGF的高表达相关，表明与外源性酸性FGF相反，内源性酸性FGF不是促有丝分裂因子，而是存活因子。
