Maier J A, Morelli D, Ménard S, Colnaghi M I, Balsari A
Department of Biomedical Sciences and Technologies, Ospedale San Raffaele, Milan, Italy.
Am J Pathol. 1996 Sep;149(3):945-52.
Endothelial cells undergo apoptosis after withdrawal of growth factors, alterations in the extracellular matrix, or exposure to cytokines. Here we report that tumor necrosis factor (TNF)-alpha induces apoptosis of human endothelial cells derived from the umbilical vein in a dose-dependent fashion. Apoptosis is triggered through a pathway that is independent from the levels of Bcl-2. On the contrary, TNF stimulates the growth of spontaneously transformed human umbilical vein endothelial cells. This proliferative effect is mediated through the up-regulation of fibroblast growth factor-1 by TNF. The addition of specific fibroblast growth factor-1 antisense oligonucleotides inhibits TNF-induced fibroblast growth factor-1 expression, thus inhibiting the growth and triggering apoptosis of spontaneously transformed human umbilical vein endothelial cells.
在生长因子撤除、细胞外基质改变或暴露于细胞因子后，内皮细胞会发生凋亡。在此我们报告，肿瘤坏死因子（TNF）-α以剂量依赖方式诱导人脐静脉来源的内皮细胞凋亡。凋亡是通过一条独立于Bcl-2水平的途径触发的。相反，TNF刺激自发转化的人脐静脉内皮细胞生长。这种增殖效应是通过TNF上调成纤维细胞生长因子-1介导的。添加特异性成纤维细胞生长因子-1反义寡核苷酸可抑制TNF诱导的成纤维细胞生长因子-1表达，从而抑制自发转化的人脐静脉内皮细胞的生长并触发其凋亡。
