Farmer B T, Müller L, Nikonowicz E P, Pardi A
Pharmaceutical Research Institute, Bristol-Myers Squibb, Princeton, NJ 084543-4000.
J Biomol NMR. 1994 Jan;4(1):129-33. doi: 10.1007/BF00178341.
A set of three 3D (1H,13C,15N) triple-resonance correlation experiments has been designed to provide H1'-H8 intraresidue sugar-to-base correlations in purines in an unambiguous and efficient manner. Together, the HsCsNb, HsCs(N)bCb, and HbNbCb experiments correlate the H1' sugar proton to the H8 proton of the attached base by means of the (H1', C1', N9, C8, H8) heteronuclear scalar coupling network. The assignment strategy presented here allows for unambiguous H1'-H8 intraresidue correlations, provided that no two purines have both the same H1' and C1' chemical shifts and the same C8 and N9 chemical shifts. These experiments have yielded H1'-H8 intraresidue sugar-to-base correlations for all five guanosines in the [13C,15N] isotopically labeled RNA duplex r(GGCGCUUGCGUC)2.
设计了一组三个3D（1H，13C，15N）三共振相关实验，以明确且高效地提供嘌呤中H1'-H8残基内糖与碱基的相关性。HsCsNb、HsCs(N)bCb和HbNbCb实验共同通过（H1'，C1'，N9，C8，H8）异核标量耦合网络，将H1'糖质子与连接碱基的H8质子相关联。只要没有两个嘌呤具有相同的H1'和C1'化学位移以及相同的C8和N9化学位移，此处提出的归属策略就能实现明确的H1'-H8残基内相关性。这些实验已在[13C，15N]同位素标记的RNA双链体r(GGCGCUUGCGUC)2中，得出了所有五个鸟苷的H1'-H8残基内糖与碱基的相关性。
