Sklenár V, Dieckmann T, Butcher S E, Feigon J
Department of Physical and Theoretical Chemistry, Masaryk University, Brno, Czech Republic.
J Biomol NMR. 1996 Jan;7(1):83-7. doi: 10.1007/BF00190460.
Novel HCCNH TOCSY NMR experiments are presented that provide unambiguous assignment of the exchangeable imino proton resonances by intranucleotide through-bond connectivities to the (assigned) nonexchangeable purine H8 and pyrimidine H6 protons in uniformly 15N-, 13C-labeled RNA oligonucleotides. The HCCNH TOCSY experiments can be arranged as a two-dimensional experiment, correlating solely GH8/UH6 and GH1/UH3 proton resonances (HCCNH), 51 as three-dimensional experiments, in which additional chemical shift labeling either by GN1/UN3 (HCCNH) or by GC8/UC6 (HCCNH) chemical shifts is introduced. The utility of these experiments for the assignment of relatively large RNA oligonucleotides is demonstrated for two different RNA molecules.
本文介绍了新型的HCCNH TOCSY NMR实验，该实验通过核苷酸内的键间连接，将均匀15N、13C标记的RNA寡核苷酸中可交换的亚氨基质子共振明确地指定给（已指定的）不可交换的嘌呤H8和嘧啶H6质子。HCCNH TOCSY实验可以设置为二维实验，仅关联GH8/UH6和GH1/UH3质子共振（HCCNH），也可以设置为三维实验，其中引入了通过GN1/UN3（HCCNH）或GC8/UC6（HCCNH）化学位移进行的额外化学位移标记。针对两个不同的RNA分子，展示了这些实验在相对较大的RNA寡核苷酸归属方面的实用性。
