Olsen P B, Klemm P
Department of Microbiology, Technical University of Denmark, Lyngby.
FEMS Microbiol Lett. 1994 Feb 1;116(1):95-100. doi: 10.1111/j.1574-6968.1994.tb06681.x.
The expression of type 1 fimbriae in Escherichia coli undergoes phase variation in which individual bacteria switch between a fimbriated and non-fimbriated state. The transition from one state to the other is caused by inversion of a DNA segment containing the promoter for the fimA gene. The orientation of the invertible segment is controlled by two proteins, FimB and FimE, which mediate an on/off and off only orientation of the segment, respectively. In this study we have mapped the 5' termini of the fimB, fimE and fimA transcripts. Furthermore, we show that expression of fimB and fimE is strongly influenced by the H-NS nucleoid protein.
大肠杆菌中1型菌毛的表达会发生相变，在此过程中单个细菌在菌毛化和非菌毛化状态之间切换。从一种状态到另一种状态的转变是由包含fimA基因启动子的DNA片段倒位引起的。可倒位片段的方向由两种蛋白质FimB和FimE控制，它们分别介导该片段的开启/关闭和仅关闭方向。在本研究中，我们绘制了fimB、fimE和fimA转录本的5'末端图谱。此外，我们表明fimB和fimE的表达受到H-NS类核蛋白的强烈影响。
