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鉴定一种定位于膜区室的角蛋白相关蛋白。

Identification of a keratin-associated protein that localizes to a membrane compartment.

作者信息

Chou C F, Riopel C L, Omary M B

机构信息

Palo Alto Veterans Administration Medical Center, CA 94304.

出版信息

Biochem J. 1994 Mar 1;298 ( Pt 2)(Pt 2):457-63. doi: 10.1042/bj2980457.

DOI:10.1042/bj2980457
PMID:7510951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1137962/
Abstract

We describe the characterization of an acidic glycoprotein (molecular mass approximately 85 kDa) that associates with keratin intermediate filaments of 'simple'-type epithelia. Using a number of anti-keratin monoclonal antibodies, the 85 kDa glycoprotein was identified by co-immunoprecipitation with keratin polypeptides 8 and 18 (K8/18) from the human colonic epithelial cell line HT29 and several other epithelial cell lines. This Keratin-Associated Protein (termed KAP85) was readily detected after in vitro galactosylation of K8/18 immunoprecipitates obtained from mitosis-arrested cells. Its solubilization and detection were dependent on the detergent used, and it was barely detected after in vitro galactosylation of asynchronously growing G0/G1-phase cells. Its poor in vitro galactosylation in G0/G1-phase cells is likely a reflection of the lack of available terminal N-acetylglucosamine residues, since it can be labelled to a similar extent in G0/G1- and G2/M-phase cells using NaIO4/NaB3H4. Glycosidase digestion showed that KAP85 contains high mannose and complex oligosaccharides. Fractionation of total cellular K8/18 into soluble and cytoskeletal insoluble pools showed that KAP85 associates exclusively with the cytoskeletal K8/18 pool. Subcellular fractionation showed that KAP85 co-localizes with a plasma-membrane-enriched fraction that includes the transferrin receptor and KS-1 antigen. Our results demonstrate in vitro evidence of a membrane-associated glycoprotein (KAP85) which may serve as an attachment site for filamentous K8/18.

摘要

我们描述了一种酸性糖蛋白（分子量约85 kDa）的特性，该糖蛋白与“简单”型上皮细胞的角蛋白中间丝相关。使用多种抗角蛋白单克隆抗体，通过与人结肠上皮细胞系HT29和其他几种上皮细胞系中的角蛋白多肽8和18（K8/18）进行共免疫沉淀，鉴定出了85 kDa的糖蛋白。这种角蛋白相关蛋白（称为KAP85）在从有丝分裂停滞细胞中获得的K8/18免疫沉淀物进行体外半乳糖基化后很容易被检测到。其溶解和检测取决于所用的去污剂，在异步生长的G0/G1期细胞的体外半乳糖基化后几乎检测不到。它在G0/G1期细胞中体外半乳糖基化较差可能反映了缺乏可用的末端N-乙酰葡糖胺残基，因为使用NaIO4/NaB3H4在G0/G1期和G2/M期细胞中它可以被标记到相似的程度。糖苷酶消化表明KAP85含有高甘露糖和复合寡糖。将总细胞K8/18分离为可溶性和细胞骨架不溶性部分表明，KAP85仅与细胞骨架K8/18部分相关。亚细胞分级分离表明，KAP85与富含质膜的部分共定位，该部分包括转铁蛋白受体和KS-1抗原。我们的结果证明了一种膜相关糖蛋白（KAP85）的体外证据，它可能作为丝状K8/18的附着位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/be1764ee9247/biochemj00092-0209-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/e4538b3a4236/biochemj00092-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/70504c512d33/biochemj00092-0207-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/1198f8ea01e6/biochemj00092-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/cce5d1a392f8/biochemj00092-0208-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/a145a6c1f70b/biochemj00092-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/25a188885a9b/biochemj00092-0209-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/be1764ee9247/biochemj00092-0209-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/e4538b3a4236/biochemj00092-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/70504c512d33/biochemj00092-0207-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/1198f8ea01e6/biochemj00092-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/cce5d1a392f8/biochemj00092-0208-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/a145a6c1f70b/biochemj00092-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/25a188885a9b/biochemj00092-0209-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70b5/1137962/be1764ee9247/biochemj00092-0209-c.jpg

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本文引用的文献

1
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Science. 1993 Apr 30;260(5108):687-9. doi: 10.1126/science.8480179.
2
Desmosomes and hemidesmosomes.桥粒和半桥粒。
Curr Opin Cell Biol. 1993 Feb;5(1):30-40. doi: 10.1016/s0955-0674(05)80005-5.
3
Method of cell handling affects leakiness of cell surface labeling and detection of intracellular keratins.细胞处理方法会影响细胞表面标记的渗漏以及细胞内角蛋白的检测。
角蛋白20在兔十二指肠的内分泌和外分泌细胞中表达。
Acta Histochem Cytochem. 2007 Aug 30;40(4):123-30. doi: 10.1267/ahc.07007.
4
Identification of novel principles of keratin filament network turnover in living cells.活细胞中角蛋白丝网络周转新原理的鉴定。
Mol Biol Cell. 2004 May;15(5):2436-48. doi: 10.1091/mbc.e03-09-0707. Epub 2004 Mar 5.
5
14-3-3 proteins associate with phosphorylated simple epithelial keratins during cell cycle progression and act as a solubility cofactor.在细胞周期进程中，14-3-3蛋白与磷酸化的简单上皮角蛋白结合，并作为一种溶解性辅助因子发挥作用。
J Cell Biol. 1996 Apr;133(2):345-57. doi: 10.1083/jcb.133.2.345.
6
Dynamics of human keratin 18 phosphorylation: polarized distribution of phosphorylated keratins in simple epithelial tissues.人角蛋白18磷酸化的动力学：磷酸化角蛋白在单层上皮组织中的极化分布。
J Cell Biol. 1995 Dec;131(5):1291-301. doi: 10.1083/jcb.131.5.1291.
Cell Motil Cytoskeleton. 1993;26(1):77-87. doi: 10.1002/cm.970260108.
4
A significant soluble keratin fraction in 'simple' epithelial cells. Lack of an apparent phosphorylation and glycosylation role in keratin solubility.“简单”上皮细胞中存在显著的可溶性角蛋白组分。角蛋白溶解性中缺乏明显的磷酸化和糖基化作用。
J Cell Sci. 1993 Jun;105 ( Pt 2):433-44. doi: 10.1242/jcs.105.2.433.
5
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6
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Cell. 1993 Feb 26;72(4):561-74. doi: 10.1016/0092-8674(93)90075-2.
7
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J Biol Chem. 1980 Feb 25;255(4):1662-9.
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10
The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.人类细胞角蛋白目录：在正常上皮、肿瘤及培养细胞中的表达模式
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