Khosla J, Correa M T, Sannes P L
Department of Anatomy, Physiological Sciences, and Radiology, North Carolina State University, College of Veterinary Medicine, Raleigh 27606.
Am J Respir Cell Mol Biol. 1994 Apr;10(4):462-9. doi: 10.1165/ajrcmb.10.4.7510987.
The purpose of this study was to determine whether the cytochemically defined distribution of sulfated macromolecules is significantly different in microdomains of basement membranes (BMs) associated with different levels of pulmonary airways. The high iron diamine-thiocarbohydrazide-silver proteinate (HID-TCH-SP) technique, which is highly specific for sulfate esters of glycosaminoglycans and some glycoproteins, was used as a probe to compare the BM of trachea, bronchi, and three different sizes of bronchioles. When HID-reactive sites were counted and statistically compared, significant differences were found between the three known anatomically distinct layers of the BM--lamina lucida (LL), lamina densa (LD), and lamina reticularis (LR)--relative to the airway level. The highest concentration of HID reactivity in trachea, bronchi, and large bronchiole was found in LR and the lowest in LD. By comparison, HID-reactive sites were found to be more concentrated in the LL in medium and small bronchioles. HID reactivity was consistently low in LD as compared with LL and LR in all five locations. The overall degree of HID reactivity in BMs was clearly highest in large bronchioles and lowest in medium and small bronchioles. This cytochemically detectable heterogeneity in the distribution of HID reactivity in BM microdomains may represent specific compositional differences in pulmonary BMs which are important determinants of epithelial cell function and might be expected to impact key biologic processes in normal and pathologic states.
本研究的目的是确定与不同水平的肺气道相关的基底膜（BMs）微区中硫酸化大分子的细胞化学定义分布是否存在显著差异。高铁二胺 - 硫代碳酰肼 - 银蛋白技术（HID - TCH - SP）对糖胺聚糖和一些糖蛋白的硫酸酯具有高度特异性，被用作探针来比较气管、支气管和三种不同大小细支气管的基底膜。当对HID反应位点进行计数并进行统计学比较时，发现相对于气道水平，基底膜的三个已知解剖学上不同的层——透明层（LL）、致密层（LD）和网状层（LR）——之间存在显著差异。在气管、支气管和大细支气管中，HID反应性的最高浓度出现在LR，最低浓度出现在LD。相比之下，在中细支气管和小细支气管中，HID反应位点在LL中更为集中。在所有五个位置，与LL和LR相比，LD中的HID反应性始终较低。基底膜中HID反应性的总体程度在大细支气管中明显最高，在中细支气管和小细支气管中最低。基底膜微区中HID反应性分布的这种可通过细胞化学检测到的异质性可能代表肺基底膜的特定组成差异，这些差异是上皮细胞功能的重要决定因素，并且可能会影响正常和病理状态下的关键生物学过程。
