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通过二维P(CC)H-TOCSY三重共振核磁共振实验对均匀13C标记的RNA进行序列主链归属。

Sequential backbone assignment of uniformly 13C-labeled RNAs by a two-dimensional P(CC)H-TOCSY triple resonance NMR experiment.

作者信息

Wijmenga S S, Heus H A, Leeuw H A, Hoppe H, van der Graaf M, Hilbers C W

机构信息

SON/NWO National HF-NMR Facility, Nijmegen, The Netherlands.

出版信息

J Biomol NMR. 1995 Jan;5(1):82-6. doi: 10.1007/BF00227472.

DOI:10.1007/BF00227472
PMID:7533569
Abstract

A new 1H-13C-31P triple resonance experiment is described which allows unambiguous sequential backbone assignment in 13C-labeled oligonucleotides via through-bond coherence transfer from 31P via 13C to 1H. The approach employs INEPT to transfer coherence from 31P to 13C and homonuclear TOCSY to transfer the 13C coherence through the ribose ring, followed by 13C to 1H J-cross-polarisation. The efficiencies of the various possible transfer pathways are discussed. The most efficient route involves transfer of 31Pi coherence via C4'i and C4'i-1, because of the relatively large JPC4' couplings involved. Via the homonuclear and heteronuclear mixing periods, the C4'i and C4'i-1 coherences are subsequently transferred to, amongst others, H1'i and H1'i-1, respectively, leading to a 2D 1H-31P spectrum which allows a sequential assignment in the 31P-1H1' region of the spectrum, i.e. in the region where the proton resonances overlap least. The experiment is demonstrated on a 13C-labeled RNA hairpin with the sequence 5'(GGGC-CAAA-GCCU)3'.

摘要

本文描述了一种新的1H-13C-31P三共振实验，该实验可通过从31P经13C到1H的键间相干转移，在13C标记的寡核苷酸中实现明确的主链序列归属。该方法采用INEPT将相干从31P转移到13C，利用同核TOCSY将13C相干通过核糖环进行转移，随后进行13C到1H的J交叉极化。文中讨论了各种可能转移途径的效率。最有效的途径是通过C4'i和C4'i-1转移31Pi相干，这是因为涉及的JPC4'耦合相对较大。通过同核和异核混合期，C4'i和C4'i-1相干随后分别转移到H1'i和H1'i-1等，从而得到二维1H-31P谱，该谱允许在谱的31P-1H1'区域进行序列归属，即在质子共振重叠最少的区域。在一个序列为5'(GGGC-CAAA-GCCU)3'的13C标记RNA发夹上展示了该实验。

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本文引用的文献

1
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Biochemistry. 1993 Jan 19;32(2):395-400. doi: 10.1021/bi00053a002.
2
An efficient procedure for assignment of the proton, carbon and nitrogen resonances in 13C/15N labeled nucleic acids.一种用于确定¹³C/¹⁵N标记核酸中质子、碳和氮共振峰归属的有效方法。
J Mol Biol. 1993 Aug 20;232(4):1141-56. doi: 10.1006/jmbi.1993.1466.
3
Two- and three-dimensional 31P-driven NMR procedures for complete assignment of backbone resonances in oligodeoxyribonucleotides.
J Biomol NMR. 1996 Sep;8(2):147-60. doi: 10.1007/BF00211161.
4
A New Experiment for the Measurement of nJ(C,P) Coupling Constants Including 3J(C4'i,Pi) and 3J(C4'i,Pi+1) in Oligonucleotides.寡核苷酸中 nJ(C,P)耦合常数的新实验测量，包括 3J(C4'i,Pi)和 3J(C4'i,Pi+1)。
J Biomol NMR. 1998 Aug;12(2):223-30. doi: 10.1023/A:1008258226683.
5
Preparation of selective and segmentally labeled single-stranded DNA for NMR by self-primed PCR and asymmetrical endonuclease double digestion.通过自引发聚合酶链反应和不对称核酸内切酶双酶切制备用于核磁共振的选择性和片段标记的单链DNA
Nucleic Acids Res. 2009 Sep;37(17):e114. doi: 10.1093/nar/gkp540. Epub 2009 Jun 24.
6
A complete set of novel 2D correlation NMR experiments based on heteronuclear J-cross polarization.基于异核J交叉极化的一套完整的新型二维相关核磁共振实验。
J Biomol NMR. 2004 May;29(1):37-55. doi: 10.1023/B:JNMR.0000019514.04047.8b.
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J Magn Reson B. 1994 Feb;103(2):134-41. doi: 10.1006/jmrb.1994.1021.
5
Unambiguous through-bond sugar-to-base correlations for purines in 13C,15N-labeled nucleic acids: the HsCsNb,HsCs(N)bCb, and HbNbCb experiments.13C、15N标记核酸中嘌呤的明确的糖-碱基通过键相关性：HsCsNb、HsCs(N)bCb和HbNbCb实验
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6
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8
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9
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10
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