Ueda S, Gu X F, Whitaker-Azmitia P M, Naruse I, Azmitia E C
Department of Biology, New York University, NY 10003.
Brain Res. 1994 Jan 7;633(1-2):275-83. doi: 10.1016/0006-8993(94)91549-0.
The homozygote of a mouse strain with genetic polydactyly (Polydactyly Nagoya; Pdn) shows several brain abnormalities, and significant decrease of S-100 beta in the brain. In order to clarify the effects of the retarded production of S-100 beta on the development of monoaminergic neuronal systems and supporting glial cells, immunocytochemical studies of tyrosine hydroxylase (TH), serotonin (5-HT), S-100 beta and glial fibrillary acidic protein (GFAP). In addition, high-performance liquid-chromatography (HPLC) measurements of serotonin and 5-hydroxyindoleacetic acid (5-HIAA) of homozygote (Pdn/Pdn) mouse were examined, and the results were compared with those of other genotypes; heterozygote (Pdn/+) and wild type (+/+) mice. In all types of mice, S-100 beta positive cells and serotonergic fibers were widely distributed throughout the brains and serotonergic cell bodies were located in the brainstem. However, the hippocampus and caudo-dorsal cortex of Pdn/Pdn mouse were markedly reduced in S-100 beta positive cells and in serotonergic fibers. Furthermore, abnormal distribution of GFAP positive cells and fibers were observed in the neocortex and hippocampus of Pdn/Pdn brain. No differences were seen in the distribution of TH neurons or fibers distribution. In the HPLC study, the content of 5-HT and 5-HIAA of the hippocampus and cortex of Pdn/Pdn mouse was lower than those of Pdn/+ and +/+ mice. The present results suggest that the developmental defect of serotonergic fibers in the Pdn mutant mouse is correlate to the deficiency of S-100 beta in the astrocyte of this mutant.
具有遗传性多指（多指名古屋；Pdn）的小鼠品系纯合子表现出多种脑部异常，且脑中S-100β显著减少。为了阐明S-100β产生延迟对单胺能神经元系统和支持性神经胶质细胞发育的影响，进行了酪氨酸羟化酶（TH）、血清素（5-HT）、S-100β和胶质纤维酸性蛋白（GFAP）的免疫细胞化学研究。此外，检测了纯合子（Pdn/Pdn）小鼠血清素和5-羟吲哚乙酸（5-HIAA）的高效液相色谱（HPLC）测量值，并将结果与其他基因型进行比较；杂合子（Pdn/+）和野生型（+/+）小鼠。在所有类型的小鼠中，S-100β阳性细胞和血清素能纤维广泛分布于整个大脑，血清素能细胞体位于脑干。然而，Pdn/Pdn小鼠的海马体和尾背皮质中S-100β阳性细胞和血清素能纤维明显减少。此外，在Pdn/Pdn脑的新皮质和海马体中观察到GFAP阳性细胞和纤维的异常分布。TH神经元的分布或纤维分布没有差异。在HPLC研究中，Pdn/Pdn小鼠海马体和皮质中5-HT和5-HIAA的含量低于Pdn/+和+/+小鼠。目前的结果表明，Pdn突变小鼠中血清素能纤维的发育缺陷与该突变体星形胶质细胞中S-100β的缺乏有关。
