Knuechel R, Meckl S, Masters J R, Hofstaedter F
Universität Regensburg.
Verh Dtsch Ges Pathol. 1993;77:256-9.
The distribution of four antibodies against urothelial-associated glycoproteins (UAGAb: Uro1, -5, -9, -10, SIGNET) was studied by dual parameter flow-cytometry (UAGAb and DNA staining) in normal non-urothelial cancer cell lines (2), normal urothelial cell lines (2) and bladder cell lines (5). When cell lines were compared as plateau monolayers, high proportions of Uro1 and Uro10 were found in all cell lines, in contrast to Uro5 and Uro9, both showing specificity for urothelial cells. Though being described as a pan-urothelial marker, Uro5 is not found in all cell lines. In two cell lines (RT4 and J82) three different growth states (exponential and plateau monolayer and multicellular spheroids were compared, and dependence on spatial configuration could be shown for Uro9. Cell cycle relation of expression was found for Uro1 and Uro10.
通过双参数流式细胞术（尿路上皮相关糖蛋白抗体染色和DNA染色）研究了四种抗尿路上皮相关糖蛋白抗体（UAGAb：Uro1、-5、-9、-10、SIGNET）在正常非尿路上皮癌细胞系（2种）、正常尿路上皮细胞系（2种）和膀胱癌细胞系（5种）中的分布情况。当将细胞系作为平台单层进行比较时，所有细胞系中均发现高比例的Uro1和Uro10，而Uro5和Uro9则不同，二者均显示对尿路上皮细胞具有特异性。尽管Uro5被描述为泛尿路上皮标志物，但并非在所有细胞系中都能检测到。在两种细胞系（RT4和J82）中，比较了三种不同生长状态（指数生长期、平台单层和多细胞球体），结果显示Uro9的表达依赖于空间构型。还发现了Uro1和Uro10的表达与细胞周期的关系。
