Karamitopoulou E, Perentes E, Diamantis I, Maraziotis T
Sandoz Pharma Ltd., Department of Toxicology, Basle, Switzerland.
Acta Neuropathol. 1994;87(1):47-54. doi: 10.1007/BF00386253.
Paraffin-embedded surgical specimens from 136 primary human central nervous system (CNS) tumors, including 50 meningiomas, 24 astrocytomas, 26 anaplastic astrocytomas, 9 glioblastomas, 8 oligodendrogliomas, 4 ependymomas, 1 anaplastic ependymoma, 2 subependymomas, 9 medulloblastomas, and 3 paragangliomas, were immunostained, following microwave processing, using a streptavidin/peroxidase method and the MIB 1 monoclonal antibody (mAb) against the Ki-67 antigen. The following mean Ki-67 labeling index (LI) values +/- SD were found: meningiomas, 2.47 +/- 1.83; astrocytomas, 2.03 +/- 2.03; anaplastic astrocytomas, 12.80 +/- 6.29; glioblastomas, 14.57 +/- 6.77; oligodendrogliomas, 5.06 +/- 4.78; ependymomas, 2.63 +/- 2.58; anaplastic ependymoma, 6.89; subependymomas, 1.79 +/- 1.54; medulloblastomas, 18.77 +/- 9.65; and paragangliomas, 2.19 +/- 2.51. Our findings indicate that while malignant CNS tumors always exhibited high Ki-67 LI values, and benign CNS tumors generally displayed lower values, increased immunoreactivity for Ki-67 epitopes (Ki-67 LI higher than 4) was noted in a number of meningiomas, astrocytomas, ependymomas, oligodendrogliomas and paragangliomas, contrasting with their benign histological features. Further investigations of the Ki-67 immunoreactivity in CNS tumors and systematic correlation with the postoperative follow-up of patients are necessary to determine the value of Ki-67 LI in predicting the biological behavior of CNS neoplasms.
对136例原发性人类中枢神经系统（CNS）肿瘤的石蜡包埋手术标本进行免疫染色，这些肿瘤包括50例脑膜瘤、24例星形细胞瘤、26例间变性星形细胞瘤、9例胶质母细胞瘤、8例少突胶质细胞瘤、4例室管膜瘤、1例间变性室管膜瘤、2例室管膜下瘤、9例髓母细胞瘤和3例副神经节瘤。采用链霉亲和素/过氧化物酶法和抗Ki-67抗原的MIB 1单克隆抗体（mAb），经微波处理后进行免疫染色。发现以下平均Ki-67标记指数（LI）值±标准差：脑膜瘤，2.47±1.83；星形细胞瘤，2.03±2.03；间变性星形细胞瘤，12.80±6.29；胶质母细胞瘤，14.57±6.77；少突胶质细胞瘤，5.06±4.78；室管膜瘤，2.63±2.58；间变性室管膜瘤，6.89；室管膜下瘤，1.79±1.54；髓母细胞瘤，18.77±9.65；副神经节瘤，2.19±2.51。我们的研究结果表明，虽然恶性中枢神经系统肿瘤总是表现出高Ki-67 LI值，而良性中枢神经系统肿瘤通常显示较低的值，但在一些脑膜瘤、星形细胞瘤、室管膜瘤、少突胶质细胞瘤和副神经节瘤中，观察到Ki-67表位的免疫反应性增加（Ki-67 LI高于4），这与其良性组织学特征形成对比。有必要进一步研究中枢神经系统肿瘤中的Ki-67免疫反应性，并将其与患者术后随访进行系统关联，以确定Ki-67 LI在预测中枢神经系统肿瘤生物学行为方面的价值。
