Skjulsvik Anne J, Mørk Jørgen N, Torp Morten O, Torp Sverre H
Department of Laboratory Medicine, Children's and Women's Health, Faculty of Medicine, Norwegian University of Science and Technology (NTNU) Trondheim, Norway ; Department of Pathology and Medical Genetics, St. Olavs Hospital, Trondheim University Hospital Trondheim, Norway.
Department of Laboratory Medicine, Children's and Women's Health, Faculty of Medicine, Norwegian University of Science and Technology (NTNU) Trondheim, Norway.
Int J Clin Exp Pathol. 2014 Dec 1;7(12):8905-10. eCollection 2014.
Histopathological malignancy grading of human gliomas is limited by subjective interpretation of the morphological criteria. Assessment of mitotic activity is a cornerstone of grading these tumours, but mitotic figures can be hard to identify in haematoxylin-eosin stained sections. Thus, determining proliferative activity by means of Ki-67/MIB-1 immunostaining has become a useful supplement. However, this method has drawbacks, so continuous testing and evaluation are required for optimization and standardization. The aim of this study was to analyse and evaluate the Ki-67/MIB-1 proliferative indices (PIs) in a series of gliomas. We found that Ki-67/MIB-1 PIs correlated well with histological malignancy grade in all glioma subtypes, but a considerable overlap of PIs was observed between the malignancy groups. Consequently, Ki-67/MIB-1 immunostaining alone is not sufficient to adequately determine the malignancy grade. Therefore, future work is necessary to clarify the role of this immunostaining in the histopathological diagnosis of human gliomas.
人类胶质瘤的组织病理学恶性分级受形态学标准主观解读的限制。有丝分裂活性评估是这些肿瘤分级的基石，但在苏木精-伊红染色切片中，有丝分裂象可能难以识别。因此，通过Ki-67/MIB-1免疫染色来确定增殖活性已成为一种有用的补充方法。然而，该方法存在缺点，因此需要持续测试和评估以进行优化和标准化。本研究的目的是分析和评估一系列胶质瘤中的Ki-67/MIB-1增殖指数（PIs）。我们发现，在所有胶质瘤亚型中，Ki-67/MIB-1 PIs与组织学恶性分级密切相关，但在恶性组之间观察到PIs有相当大的重叠。因此，仅靠Ki-67/MIB-1免疫染色不足以充分确定恶性分级。所以，未来有必要开展工作以阐明这种免疫染色在人类胶质瘤组织病理学诊断中的作用。
