Taga T, Kariya Y, Shimada M, Uchida A
Department of Late Effect Studies, Kyoto University, Japan.
Immunol Lett. 1993 Dec;39(1):65-70. doi: 10.1016/0165-2478(93)90165-x.
Patients with aplastic anemia were tested for natural killer (NK) activity, and the roles of granulocytes and granulocyte colony-stimulating factor (G-CSF) in the regulation of cytotoxicity were evaluated. Blood lymphocytes showed low or no NK activity against K562 targets. The depression of NK activity was more frequently recorded for patients who were not in remission and those who received G-CSF administration. Granulocytes of aplastic anemia patients with impaired NK activity suppressed the lytic activity of NK cells. By contrast, granulocytes from normal controls and aplastic anemia patients with normal NK activity had no suppressive activity. There was a good correlation between NK activity of lymphocytes and suppressive activity of granulocytes. Blocking of direct contact of suppressor and effector cells by cell chambers abolished suppression of cytotoxicity. NK suppression by granulocytes was resistant to treatment with catalase or superoxide dismutase. In vitro stimulation with G-CSF of granulocytes that naturally had no suppressive activity resulted in development of suppressive function, whereas granulocytes with natural suppressive activity were not further stimulated in vitro by G-CSF to express augmented activity. These results suggest that the presence of suppressor granulocytes in the blood could be one cause of the impaired NK activity in patients with aplastic anemia.
对再生障碍性贫血患者进行了自然杀伤（NK）活性检测，并评估了粒细胞和粒细胞集落刺激因子（G-CSF）在细胞毒性调节中的作用。血液淋巴细胞对K562靶细胞显示出低NK活性或无NK活性。未缓解的患者以及接受G-CSF治疗的患者中，NK活性降低的情况更为常见。NK活性受损的再生障碍性贫血患者的粒细胞抑制了NK细胞的裂解活性。相比之下，来自正常对照和NK活性正常的再生障碍性贫血患者的粒细胞没有抑制活性。淋巴细胞的NK活性与粒细胞的抑制活性之间存在良好的相关性。通过细胞室阻断抑制细胞和效应细胞的直接接触可消除细胞毒性的抑制作用。粒细胞对NK的抑制作用对过氧化氢酶或超氧化物歧化酶治疗具有抗性。用G-CSF体外刺激天然无抑制活性的粒细胞会导致抑制功能的发展，而具有天然抑制活性的粒细胞在体外不会被G-CSF进一步刺激以表达增强的活性。这些结果表明，血液中存在抑制性粒细胞可能是再生障碍性贫血患者NK活性受损的一个原因。
