Morikawa K, Nakano A, Oseko F, Morikawa S
First Department of Internal Medicine, Shimane Medical University, Izumo, Japan.
Jpn J Med. 1989 Sep-Oct;28(5):585-92. doi: 10.2169/internalmedicine1962.28.585.
The mechanism which produces marrow failure in idiopathic aplastic anemia is still unknown. Recent investigations have suggested the crucial role of NK cells in the regulation of normal hematopoiesis. In this study, the cytotoxic activity of mononuclear cells from human bone marrow and peripheral blood was examined against three NK-sensitive target cell lines in 15 patients with aplastic anemia as well as 21 normal subjects. Marrow mononuclear cells from aplastic anemia demonstrated a high cytotoxicity comparable to peripheral blood NK cells to these target cells. Neither large granular lymphocytes nor the cells expressing known NK cell surface phenotypes increased in aplastic marrow cell elements. The aplastic marrow cells showed strong killing activity rather than binding at single cell assay. They consisted of non-adherent and adherent cell population in plastic adherence and were unresponsive to IFN treatment. The existence of cytotoxic cells with high NK-like activity may be responsible for the mechanism of marrow failure in aplastic anemia.
特发性再生障碍性贫血导致骨髓衰竭的机制尚不清楚。最近的研究表明自然杀伤（NK）细胞在正常造血调节中起关键作用。在本研究中，检测了15例再生障碍性贫血患者及21名正常受试者的骨髓和外周血单个核细胞对三种NK敏感靶细胞系的细胞毒活性。再生障碍性贫血患者的骨髓单个核细胞对这些靶细胞表现出与外周血NK细胞相当的高细胞毒性。再生障碍性贫血骨髓细胞成分中，大颗粒淋巴细胞及表达已知NK细胞表面表型的细胞均未增加。在单细胞检测中，再生障碍性贫血骨髓细胞表现出较强的杀伤活性而非结合活性。它们在塑料贴壁培养中由非贴壁和贴壁细胞群体组成，且对干扰素治疗无反应。具有高NK样活性的细胞毒性细胞的存在可能是再生障碍性贫血骨髓衰竭机制的原因。
