Wijngaard P L, MacHugh N D, Metzelaar M J, Romberg S, Bensaid A, Pepin L, Davis W C, Clevers H C
Department of Immunology, University Hospital, Utrecht, The Netherlands.
J Immunol. 1994 Apr 1;152(7):3476-82.
CD4-CD8- gamma delta T cells of ruminants uniquely express a 220-kDa surface Ag recognized by several mAbs clustered as WC1. We recently reported the isolation of a cDNA clone encoding a WC1 Ag. Southern blotting suggested that the bovine genome contains multiple sequences highly related to the isolated WC1 cDNA. Here, we demonstrate that some of the clustered WC1 mAbs stain predominantly nonoverlapping subsets of bovine CD4-CD8- gamma delta T cells. By the isolation of two additional cDNA clones encoding molecules highly related to the original WC1 Ag, we provide a molecular basis for this phenomenon. Cells transfected with cDNAs encoding individual WC1 Ags are differentially recognized by various WC1 mAbs. Thus, expression of members of the WC1 gene family divides bovine CD4-CD8- gamma delta T cells into phenotypical subsets. Field inversion gel electrophoresis revealed that all WC1 genes map to a single, large (> 1 Mbp) Notl fragment. Although the function of WC1 remains unknown, it likely involves interaction with ligands that originate from a similarly complex genetic system.
反刍动物的CD4-CD8-γδT细胞独特地表达一种220 kDa的表面抗原，该抗原可被几种单克隆抗体识别，这些单克隆抗体归为WC1。我们最近报道了一个编码WC1抗原的cDNA克隆的分离。Southern印迹分析表明，牛基因组包含多个与分离出的WC1 cDNA高度相关的序列。在此，我们证明一些聚集的WC1单克隆抗体主要标记牛CD4-CD8-γδT细胞的非重叠亚群。通过分离另外两个编码与原始WC1抗原高度相关分子的cDNA克隆，我们为这一现象提供了分子基础。用编码单个WC1抗原的cDNA转染的细胞被各种WC1单克隆抗体以不同方式识别。因此，WC1基因家族成员的表达将牛CD4-CD8-γδT细胞分为表型亚群。脉冲场凝胶电泳显示，所有WC1基因都定位于一个单一的、大的（>1 Mbp）Notl片段上。尽管WC1的功能尚不清楚，但它可能涉及与源自类似复杂遗传系统的配体的相互作用。
