Schulte E K
Anatomical Institute, Faculty of Medicine, Johannes Gutenberg-Universität, Mainz, Germany.
Biotech Histochem. 1994 Jan;69(1):7-17. doi: 10.3109/10520299409106255.
This paper discusses the impact of both standardization and quality testing of dyes and stains in biology and medicine. After the brief review of why standardized dyes and strains are not presently available commercially, two types of testing and ways of improving dye quality are described. National or international organizations could be established to define standardization of dyes and stains. Standardization would be specifically defined as a list of physico-chemical parameters such as elaborated in this paper. Commercial batches of comparable quality may be labeled by the supplier as "standard dye," a procedure currently performed by the European Council for Clinical and Laboratory Standardization (ECCLS). Also recommended to improve dye quality is commercial dye testing by independent laboratories with subsequent certification for use. This sort of quality control is currently carried out in the United States by the Biological Stain Commission (BSC). The advantages and disadvantages of both techniques and the use of image analysis for the definition of standards are discussed. A combination of both the BSC testing protocols and the ECCLS standards should be established for extended quality control of biological dyes and stains.
本文讨论了生物学和医学中染料和染色剂标准化及质量检测的影响。在简要回顾目前商业上为何没有标准化染料和染色剂之后，描述了两种检测类型以及提高染料质量的方法。可以成立国家或国际组织来定义染料和染色剂的标准化。标准化将具体定义为本文所阐述的一系列物理化学参数。质量相当的商业批次染料可由供应商标记为“标准染料”，欧洲临床和实验室标准化委员会（ECCLS）目前就是这样做的。还建议通过独立实验室对商业染料进行检测并随后颁发使用认证来提高染料质量。美国生物染色委员会（BSC）目前正在进行这种质量控制。讨论了这两种技术的优缺点以及使用图像分析来定义标准的情况。应将BSC检测方案和ECCLS标准结合起来，以对生物染料和染色剂进行更广泛的质量控制。
