Lyon H O, De Leenheer A P, Horobin R W, Lambert W E, Schulte E K, Van Liedekerke B, Wittekind D H
Department of Pathology 134, Hvidovre Hospital, University of Copenhagen, Denmark.
Histochem J. 1994 Jul;26(7):533-44. doi: 10.1007/BF00158587.
The need for the standardization of reagents and methods used in the histology laboratory is demonstrated. After definitions of dyes, stains, and chromogenic reagents, existing standards and standards organizations are discussed. This is followed by practical instructions on how to standardize dyes and stains through the preparation of reference materials and the development of chromatographic methods. An overview is presented of the problems concerned with standardization of the Romanowsky-Giemsa stain for cytological and histological application. Finally, the problem of how to convince routine dye and stain users of the need for standardization in their histology laboratories is discussed.
证明了组织学实验室中试剂和方法标准化的必要性。在对染料、染色剂和显色试剂进行定义之后，讨论了现有的标准和标准组织。接下来是关于如何通过制备参考材料和开发色谱方法来实现染料和染色剂标准化的实用指南。概述了与用于细胞学和组织学应用的罗曼诺夫斯基-吉姆萨染色标准化相关的问题。最后，讨论了如何说服组织学实验室中常规染料和染色剂使用者认识到标准化必要性的问题。
