Fukuda K, Ohtani K, Matsumura F, Tanaka S
Department of Orthopaedic Surgery, Kinki University School of Medicine, Osaka, Japan.
Connect Tissue Res. 1993;30(1):75-83. doi: 10.3109/03008209309032931.
Keratan sulfate (KS) is one of the major glycosaminoglycans in cartilage matrix proteoglycan. We find that exogenously added KS totally blocks keratanase sensitive glycosaminoglycan release measured by radiolabeled ligand. We also find that KS release is potentially inhibited by its own presence as measured by ELISA. When KS is digested with keratanase before its addition to the medium, its inhibitory effect on KS secretion is partially blocked. Exogenously added KS promotes the accumulation of the KS epitope in the cell layer. These data suggest that KS negatively regulates its own secretion.
硫酸角质素（KS）是软骨基质蛋白聚糖中的主要糖胺聚糖之一。我们发现，通过放射性标记配体测量，外源添加的KS完全阻断了角质素酶敏感的糖胺聚糖释放。我们还发现，通过酶联免疫吸附测定法（ELISA）测量，KS的存在可能会抑制其自身的释放。当KS在添加到培养基之前用角质素酶消化时，其对KS分泌的抑制作用会部分被阻断。外源添加的KS促进了KS表位在细胞层中的积累。这些数据表明，KS对其自身的分泌具有负调节作用。
