Zanetti M, Ratcliffe A, Watt F M
J Cell Biol. 1985 Jul;101(1):53-9. doi: 10.1083/jcb.101.1.53.
We have prepared a monoclonal antibody, named MZ15, that specifically binds keratan sulfate. Immunofluorescence studies showed that the distribution of keratan sulfate in articular cartilage was not uniform: the amount of keratan sulfate increased with distance from the articular surface. Two subpopulations of chondrocytes could be distinguished after isolation from cartilage by the presence or absence of cell surface keratan sulfate. Keratan sulfate-negative chondrocytes were shown to come from the upper cartilage layers. There was therefore a direct correlation between biochemical heterogeneity of cartilage matrix and heterogeneity within the chondrocyte population. During growth in monolayer culture, superficial chondrocytes began to synthesize keratan sulfate, but the cells could still be distinguished from cultures of deep or unfractionated chondrocytes by their reduced substrate adhesiveness and tendency to remain rounded.
我们制备了一种名为MZ15的单克隆抗体，它能特异性结合硫酸角质素。免疫荧光研究表明，硫酸角质素在关节软骨中的分布并不均匀：硫酸角质素的含量随着距关节表面距离的增加而增加。从软骨中分离后，根据细胞表面硫酸角质素的有无可区分出两类软骨细胞亚群。硫酸角质素阴性的软骨细胞被证明来自软骨上层。因此，软骨基质的生化异质性与软骨细胞群体的异质性之间存在直接关联。在单层培养生长过程中，表层软骨细胞开始合成硫酸角质素，但这些细胞仍可通过其较低的底物黏附性和保持圆形的倾向与深层或未分级软骨细胞的培养物区分开来。
