Berrazueta J R, Salas E, Amado J A, Sánchez de Vega M J, Poveda J J
Department of Internal Medicine, University Hospital M. Valdecilla, Cantabria University, Santander, Spain.
Eur J Pharmacol. 1994 Jan 14;251(2-3):303-5. doi: 10.1016/0014-2999(94)90414-6.
This study investigated whether human mammary arteries express an inducible nitric oxide (NO) synthase and, if so, what its effects are on vascular tone. In human mammary artery pre-contracted with phenylephrine there was a gradual time-dependent loss of tone over an 8 h period. L-Arginine and lipopolysaccharide enhanced the rate but not the magnitude of this loss in tone, whereas NG-nitro-L-arginine, NG-monomethyl-L-arginine, dexamethasone, and polymyxin B inhibited these effects. These findings indicate that incubation of human mammary artery with lipopolysaccharide resulted in the expression of an inducible NO synthase. The induction of this enzyme in human vessels may be important in the pathogenesis of septic shock.
本研究调查了人乳动脉是否表达诱导型一氧化氮（NO）合酶，若表达，其对血管张力有何影响。在用去氧肾上腺素预收缩的人乳动脉中，在8小时内张力呈时间依赖性逐渐丧失。L-精氨酸和脂多糖提高了这种张力丧失的速率，但未改变其幅度，而NG-硝基-L-精氨酸、NG-单甲基-L-精氨酸、地塞米松和多粘菌素B则抑制了这些作用。这些发现表明，人乳动脉与脂多糖孵育会导致诱导型NO合酶的表达。该酶在人体血管中的诱导可能在感染性休克的发病机制中起重要作用。
