Fuxe K, Chadi G, Tinner B, Agnati L F, Pettersson R, David G
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Brain Res. 1994 Feb 4;636(1):131-8. doi: 10.1016/0006-8993(94)90187-2.
By means of two monoclonal antibodies specific for heparan sulfate (HS)-related epitopes, one (10E4) against native HS chains and one (3G10) against desaturated uronates, a highly regional and differential distribution of these two epitopes have been observed in the adult rat brain. The 10E4 epitope immunoreactivity (IR) is mainly found in the substantia nigra, the red nucleus and the subgranular zone of the dentate gyrus, while the 3G10 epitope IR is mainly found in the CA2 area of the hippocampal formation and the pyramdial cells in the layer V of the frontoparietal cortex. The codistribution of both types of IRs with basic fibroblast growth factor (bFGF, FGF-2) in neurons and astroglia supports the notion that heparan sulfate proteoglycans (HSPG) in the extracellular matrix may serve as a site for storage of bFGF and assist in the bFGF-induced activation of the high-affinity FGF receptors linked to astroglia and neurons in these discrete areas.
利用两种针对硫酸乙酰肝素（HS）相关表位的单克隆抗体，一种（10E4）针对天然HS链，另一种（3G10）针对去饱和糖醛酸，在成年大鼠脑中观察到这两种表位具有高度的区域差异分布。10E4表位免疫反应性（IR）主要见于黑质、红核和齿状回颗粒下区，而3G10表位IR主要见于海马结构的CA2区和额顶叶皮质V层的锥体细胞。两种IR与碱性成纤维细胞生长因子（bFGF，FGF - 2）在神经元和星形胶质细胞中的共分布支持了这样一种观点，即细胞外基质中的硫酸乙酰肝素蛋白聚糖（HSPG）可能作为bFGF的储存位点，并协助bFGF诱导这些离散区域中与星形胶质细胞和神经元相连的高亲和力FGF受体的激活。
