Peris M, Frech G C, Simpson A M, Bringaud F, Byrne E, Bakker A, Simpson L
Department of Biology, UCLA School of Medicine, University of California 90024-1662.
EMBO J. 1994 Apr 1;13(7):1664-72. doi: 10.1002/j.1460-2075.1994.tb06430.x.
The molecular mechanism of RNA editing in trypanosomatid mitochondria is an unsolved problem. We show that two classes of ribonucleoprotein complexes exist in a mitochondrial extract from Leishmania tarentolae and appear to be involved in RNA editing. The 'G' class of RNP complexes consists of 170-300 A particles which contain guide RNAs and proteins, show little terminal uridylyl transferase (TUTase) activity and exhibit an in vitro RNA editing-like activity. The 'T' class consists of approximately six RNP complexes, the endogenous RNA of which can be self-labeled with [alpha-32P]UTP. The most abundant T complex, T-IV, is visualized by electron microscopy as 80-140 A particles. This complex exhibits TUTase activity in the native gel and contains guide RNAs. Both G and T complexes are possibly involved with RNA editing in vivo. These results are a starting point for the analysis of the biochemistry of RNA editing.
锥虫线粒体中RNA编辑的分子机制是一个尚未解决的问题。我们发现，来自热带利什曼原虫的线粒体提取物中存在两类核糖核蛋白复合物，它们似乎参与了RNA编辑。“G”类RNP复合物由170 - 300 Å的颗粒组成，这些颗粒含有引导RNA和蛋白质，几乎没有末端尿苷酰转移酶（TUTase）活性，但具有体外RNA编辑样活性。“T”类由大约六种RNP复合物组成，其内源RNA可用[α-32P]UTP进行自我标记。最丰富的T复合物T-IV，通过电子显微镜观察为80 - 140 Å的颗粒。该复合物在天然凝胶中表现出TUTase活性，并含有引导RNA。G和T复合物都可能在体内参与RNA编辑。这些结果是分析RNA编辑生物化学的起点。
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