Aosai F, Yang T H, Ueda M, Yano A
Department of Medical Zoology, Nagasaki University School of Medicine, Japan.
J Parasitol. 1994 Apr;80(2):260-6.
Naturally processed peptides derived from Toxoplasma gondii (T. gondii) were acid extracted from T. gondii-infected cells and detected by cytotoxic T lymphocytes (CTL) derived from peripheral blood lymphocytes of a patient with chronic toxoplasmosis. The CTL lines were obtained by weekly in vitro stimulation with a T. gondii-infected human B cell lymphoma line, ARH, which shares HLA-A2 and -Cw4 determinants with the patient. The lytic activity of these CTL lines against T. gondii-infected ARH and ARH pulsed with fraction 29 of a reversed-phase high-performance liquid chromatography (HPLC) extract from T. gondii-infected ARH was inhibited by an anti-HLA-A, B, C monoclonal antibody (mAb) and an anti-HLA-A2 mAb. Anti-HLA-DR mAb failed to block the lytic activity. Thus, the presentation of peptides by T. gondii-infected cells for CTL is mediated by HLA-A2 molecules. Interestingly, antigen presentation of ARH pulsed with naturally processed HPLC fraction 29 peptides was not inhibited by treatment with brefeldin A. The amino acid sequence of the HLA-A2-bound peptide in fraction 29 was in part consistent with the predictive algorithm of HLA-A2-binding peptide motifs.
从弓形虫感染的细胞中酸提取源自刚地弓形虫（T. gondii）的天然加工肽，并通过慢性弓形虫病患者外周血淋巴细胞衍生的细胞毒性T淋巴细胞（CTL）进行检测。CTL系通过每周用弓形虫感染的人B细胞淋巴瘤系ARH进行体外刺激获得，ARH与该患者共享HLA - A2和 - Cw4决定簇。这些CTL系对弓形虫感染的ARH以及用来自弓形虫感染的ARH的反相高效液相色谱（HPLC）提取物的第29部分脉冲处理的ARH的裂解活性被抗HLA - A、B、C单克隆抗体（mAb）和抗HLA - A2 mAb抑制。抗HLA - DR mAb未能阻断裂解活性。因此，弓形虫感染的细胞向CTL呈递肽是由HLA - A2分子介导的。有趣的是，用布雷菲德菌素A处理后，用天然加工的HPLC第29部分肽脉冲处理的ARH的抗原呈递并未受到抑制。第29部分中与HLA - A2结合的肽的氨基酸序列部分与HLA - A2结合肽基序的预测算法一致。
