细胞毒性T淋巴细胞介导的对弓形虫感染靶细胞的裂解不会导致细胞内寄生虫死亡。
Cytotoxic T-lymphocyte-mediated lysis of Toxoplasma gondii-infected target cells does not lead to death of intracellular parasites.
作者信息
Yamashita K, Yui K, Ueda M, Yano A
机构信息
Department of Medical Zoology, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki, Nagasaki 852-8523, Japan.
出版信息
Infect Immun. 1998 Oct;66(10):4651-5. doi: 10.1128/IAI.66.10.4651-4655.1998.
CD8(+) T cells play a crucial role in the control of infection with intracellular microbes. The mechanisms underlying the CD8(+) T-cell-mediated clearance of the intracellular pathogen Toxoplasma gondii are, however, not completely understood. The effect of CD8(+) cytotoxic T-lymphocyte (CTL)-mediated lysis of host cells on the viability of intracellular T. gondii was investigated. Quantitative competitive PCR of the gene encoding T. gondii major surface antigen (SAG-1) was combined with treatment of the parasites with DNase, which removed the DNA template of nonviable parasites. The induction by CD8(+) CTLs of apoptosis in cells infected with T. gondii did not result in the reduction of live parasites, indicating that intracellular T. gondii remains alive after lysis of host cells by CTLs.
CD8(+) T细胞在控制细胞内微生物感染中发挥关键作用。然而，CD8(+) T细胞介导清除细胞内病原体刚地弓形虫的潜在机制尚未完全明确。研究了CD8(+) 细胞毒性T淋巴细胞（CTL）介导的宿主细胞裂解对细胞内刚地弓形虫活力的影响。将编码刚地弓形虫主要表面抗原（SAG-1）的基因进行定量竞争性PCR，并结合用DNase处理寄生虫，DNase可去除无活力寄生虫的DNA模板。CD8(+) CTLs诱导感染刚地弓形虫的细胞发生凋亡，但并未导致活寄生虫数量减少，这表明CTL裂解宿主细胞后，细胞内的刚地弓形虫仍存活。
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