Bulgakova V G, Grushina V A, Polin A N
Antibiot Khimioter. 1993 Jul;38(7):1-4.
Polarographic determination of the rate of endogenic respiration of the cells of Staphylococcus aureus 209P showed that the respiration activity of the cells of the strain resistant to 20 micrograms/ml of gramicidin S was 20 to 30 per cent lower than that of the sensitive strain. The rate of oxygen consumption in oxidation of NADH by the membrane preparations of the resistant cells was also 25 to 30 per cent lower. By comparison with the initial sensitive strain the activity of endogenic DPI-reductases of the intact cells and NADH-dehydrogenases of the membranes of the resistant strain was also lower. The velocity of the valine transport to the resistant cells was much lower than that of the amino acid transport to the cells of the sensitive strain. Development of gramicidin S resistance in the staphylococcal strain was likely accompanied by a decrease in the activity of the energy metabolism in the membranes.
极谱法测定金黄色葡萄球菌209P细胞的内源性呼吸速率表明，对20微克/毫升短杆菌肽S具有抗性的菌株细胞的呼吸活性比敏感菌株低20%至30%。抗性细胞的膜制剂在氧化NADH时的耗氧速率也低25%至30%。与初始敏感菌株相比，抗性菌株完整细胞的内源性DPI还原酶和膜的NADH脱氢酶的活性也较低。缬氨酸向抗性细胞的转运速度远低于氨基酸向敏感菌株细胞的转运速度。葡萄球菌菌株中短杆菌肽S抗性的产生可能伴随着膜中能量代谢活性的降低。
