Bulgakova V G, Grushina V A, Polin A N
Antibiot Khimioter. 1993 Aug-Sep;38(8-9):22-5.
Strains of Staphylococcus aureus 209P growing in the presence of 20 micrograms/ml of gramicidin S were isolated after the successive subculture on a liquid medium with increasing concentrations of the antibiotic. The resistance was stable and preserved after the subculture on media not containing the antibiotic. The development of the resistance to gramicidin S did not lower the cell sensitivity to a large number of antibiotics known as inhibitors of the cell wall synthesis, protein synthesis and RNA-polymerase reaction. There was observed the development of moderate resistance to actinomycin D but not to other antibiotics interacting with DNA. The gramicidin resistant strains were also resistant to tyrocidine, a membrane active polypeptide. By the amount of the bound gramicidin S the cells of the sensitive and resistant strains did not practically differ.
在含有20微克/毫升短杆菌肽S的条件下生长的金黄色葡萄球菌209P菌株，是在含有浓度递增的该抗生素的液体培养基上连续传代培养后分离得到的。这种抗性是稳定的，在不含该抗生素的培养基上传代培养后仍得以保留。对短杆菌肽S的抗性发展并未降低细胞对大量已知为细胞壁合成抑制剂、蛋白质合成抑制剂和RNA聚合酶反应抑制剂的抗生素的敏感性。观察到对放线菌素D产生了中度抗性，但对其他与DNA相互作用的抗生素没有抗性。耐短杆菌肽的菌株对膜活性多肽酪菌素也有抗性。敏感菌株和抗性菌株的细胞结合的短杆菌肽S的量实际上没有差异。
